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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Camargo, Anderson Carlos de Paula, Otávio Almeida Lino Todorov, Svetoslav Dimitrov Nero, Luís Augusto |
| Description | Country affiliation: Brazil Author Affiliation: Camargo AC ( Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, 36570 900, Viçosa, MG, Brazil.); de Paula OA ( Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, 36570 900, Viçosa, MG, Brazil.); Todorov SD ( Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, 36570 900, Viçosa, MG, Brazil.); Nero LA ( Departamento de Veterinária, Universidade Federal de Viçosa, Campus UFV, 36570 900, Viçosa, MG, Brazil. nero@ufv.br.) |
| Abstract | The present study aimed to assess the activity of cell-free supernatant (CFS) containing bacteriocins on the formation and maintenance of biofilms developed by Listeria monocytogenes, and the associated effect of bacteriocins and ethylene-diamine-tetra-acetic acid (EDTA) on the formed biofilm. CFS from 9 lactic acid bacteria (LAB) strains was tested for inhibitory activity against 85 L. monocytogenes isolates and 21 LAB strains. Then, 12 L. monocytogenes strains were selected based on genetic profiles and sensitivity to CFS and were subjected to an in vitro assay to assess biofilm formation in microtiter plates, considering different culture media and incubation conditions. Based on these results, 6 L. monocytogenes strains were subjected to the same in vitro procedure to assess biofilm formation, being co-inoculated with CFS. In addition, these strains were subjected to the same in vitro procedure, modified by adding the CFS after biofilm formation. Relevant decrease in biofilm formation was observed in the first experiment, but CFS added after biofilm formation did not eliminate them. CFS from Lactobacillus curvatus ET31 were selected due to its anti-biofilm activity, being associated to EDTA at different concentrations and tested for biofilm control of three strains of L. monocytogenes, using the same in vitro procedure described previously. Concentrated bacteriocin presented poor performance in eliminating formed biofilms, and EDTA concentration presented no evident interference on biofilm elimination. Twelve selected L. monocytogenes strains were positive for investigated virulence makers and negative for luxS gene, recognized as being involved in biofilm formation. Selected L. monocytogenes strains were able to produce biofilms under different conditions. CFSs have the potential to prevent biofilm formation, but they were not able to destroy already formed biofilms. Nevertheless, low concentrations of CFS combined with EDTA caused a relevant reduction in already formed biofilms, but this association was not able to eliminate them. The activity of selected CFS was demonstrated against L. monocytogenes-formed biofilms, being more effective when associated to EDTA at different concentrations. |
| File Format | HTM / HTML |
| ISSN | 02732289 |
| Issue Number | 6 |
| Journal | Applied Biochemistry and Biotechnology |
| Volume Number | 178 |
| e-ISSN | 15590291 |
| Language | English |
| Publisher | Springer |
| Publisher Date | 2016-03-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Molecular Biology Environmental Engineering Biochemistry Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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