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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Klein, Joshua S. Webster, Alexandre Gnanapragasam, Priyanthi N. P. Galimidi, Rachel P. Bjorkman, Pamela J. |
| Description | Country affiliation: United States Author Affiliation: Klein JS ( Division of Biology, California Institute of Technology, Pasadena, California 91125, USA.) |
| Abstract | OBJECTIVE: Increasing data support a role for antibody-dependent cellular cytotoxicity (ADCC) in controlling HIV-1 infection. We recently isolated a naturally occurring dimeric form of the anti-HIV-1 antibody 2G12 and found it to be significantly more potent than 2G12 monomer in neutralizing primary virus strains. However, given the unusual structure of dimeric 2G12 with two Fc regions, it was not clear whether 2G12 dimer could bind to the CD16 Fc receptor on ADCC effector cells or trigger ADCC. Here we compared the in-vitro ADCC activities of 2G12 monomer and dimer and investigated the effects of including ADCC-enhancing mutations in both forms of 2G12. METHODS: An in-vitro ADCC assay using target cells stably expressing gp160 was developed to evaluate the activities of 2G12 monomer and dimer with and without ADCC-enhancing mutations that increase the CD16-binding affinity of the 2G12 Fc region. RESULTS: Both 2G12 monomer and 2G12 dimer elicited ADCC, although the dimer showed increased potency [lower half-maximal concentration (EC(50))] in triggering ADCC, thus confirming its ability to bind CD16 and trigger ADCC. The ADCC-enhancing mutations improved the ADCC activity of 2G12 monomer more than 2G12 dimer such that their EC(50) values were nearly equal. However, no increase in nonspecific ADCC activity was observed using 2G12 IgGs with these mutations. CONCLUSION: Given the likelihood that ADCC plays a role in protecting against initial infection and/or controlling chronic infection, these data suggest 2G12 dimers and/or addition of ADCC-enhancing mutations could augment the prophylactic and/or therapeutic potential of 2G12. |
| File Format | HTM / HTML |
| ISSN | 02699370 |
| e-ISSN | 14735571 |
| Journal | AIDS |
| Issue Number | 11 |
| Volume Number | 24 |
| Language | English |
| Publisher | Lippincott Williams & Wilkins |
| Publisher Date | 2010-07-17 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Discipline Immunology Antibodies, Monoclonal Immunology Antibody-dependent Cell Cytotoxicity Hiv Antibodies Hiv Infections Hiv-1 Genetics Cell Adhesion Molecules Cells, Cultured Gpi-linked Proteins Hiv Envelope Protein Gp160 Metabolism Immunoglobulin G Mutation Protein Multimerization Receptors, Igg Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Immunology and Allergy Immunology |
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