NDLI: Comparison of three methods to concentrate Giardia cysts and Cryptosporidium oocysts from surface and drinking waters.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Karim, Helmi Sylvain, Skraber Laurence, Leblanc Lucien, Hoffmann Henry-Michel, Cauchie
Description	Country affiliation: Luxembourg Author Affiliation: Karim H ( Department of Environment and Agro-biotechnologies (EVA), Centre de Recherche Public - Gabriel Lippmann, Belvaux, Luxembourg.)
Abstract	In this study, three methods commercialized by Pall (Envirochek HV), Idexx (FiltaMax) and Whatman (Cryptest) to concentrate Giardia cysts and Cryptosporidium oocysts from surface and drinking waters were compared according to the EPA standard protocol. Twenty litres of surface and 100 litres of drinking waters were inoculated with 10(3) (oo)cysts before being concentrated by filtration. Our results show that recovery rates of Giardia cysts from surface water was significantly higher using FiltaMax (91+/-12%) than Cryptest (57+/-9%) or Envirochek HV (60+/-4%) while recovery from drinking water was equivalent using FiltaMax (84+/-7%) or Cryptest (78+/-7%) but lower using Envirochek HV (34+/-29%). Recovery of Cryptosporidium oocysts from surface water was significantly higher using Envirochek HV (65+/-5%) than using FiltaMax (45+/-7%) or Cryptest (50+/-7%) while recovery rates from drinking water was equivalent using either FiltaMax (48+/-8%), Cryptest (57+/-4%) or Envirochek HV (64+/-22%). Finally, regardless of the water type, all methods tested allowed recovery rates superior to 24% complying with the EPA standard protocol. Timing, material and practicability associated with each method are discussed.
File Format	HTM / HTML
ISSN	02731223
Issue Number	1
Volume Number	62
e-ISSN	19969732
Journal	Water Science & Technology
Language	English
Publisher	IWA Publishing
Publisher Date	2010-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Hydrology Cryptosporidium Parvum Isolation & Purification Giardia Lamblia Oocysts Cytology Parasitology Methods Water Filtration Water Supply Comparative Study Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Environmental Engineering Water Science and Technology

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