NDLI: Pyrosequencing estimates of the diversity of antibiotic resistant bacteria in a wastewater system.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Sigala, Jesus Unc, Adrian
Description	Country affiliation: United States Author Affiliation: Sigala J ( Plant and Environmental Sciences, New Mexico State University, Box 30003 MSC 3Q, Skeen Hall Room N 127, Las Cruces, NM 88003-8003, USA. aunc@nmsu.edu)
Abstract	Standard protocols for monitoring wastewater treatment efficacy target Escherichia coli and fecal coliforms. This might not accurately describe risks associated with antibiotic resistance in the bacterial population of treated wastewaters. We modified a standard agar recovery method by amending it with various antibiotics. The resulting bacterial colonies were submitted to 454 pyrosequencing; thus we identified the diversity of culturable antibiotic resistant bacteria from treated and raw wastewaters. This approach produced 209,706 high quality reads of >300 bp. Operational taxonomic units (OTUs) within Proteobacteria dominated the system. The Shannon-Wiener H' index showed bacterial populations recovered on ciprofloxacin amended agars to be the least diverse. Principal component analysis of OTU distribution at phylum level showed that Proteobacteria accounted for most of the variability. The same analysis revealed most of the samples to have similar diversities at phylum level being dominated by Proteobacteria, though a few samples, typically recovered from ciprofloxacin or doxycycline amended agars were often dissimilar. Arcobacter spp. or E. coli were dominant in the bacterial communities recovered on agars amended with ciprofloxacin or doxycycline, respectively. Genera containing putative pathogens were mostly representatives of Gamma and Epsilon proteobacteria. Bacterial populations containing multiple antibiotic resistance (MAR) in the final treated effluent was a possibility.
File Format	HTM / HTML
ISSN	02731223
Issue Number	7
Volume Number	67
e-ISSN	19969732
Journal	Water Science & Technology
Language	English
Publisher	IWA Publishing
Publisher Date	2013-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Hydrology Drug Resistance, Bacterial Proteobacteria Waste Water Microbiology Water Microbiology Biodiversity Culture Techniques Principal Component Analysis Sequence Analysis, Dna Water Purification Standards Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Environmental Engineering Water Science and Technology

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