NDLI: Characteristics of hydrocarbon hydroxylase genes in a thermophilic aerobic biological system treating oily produced wastewater.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liu, Ruyin Gao, Yingxin Ji, Yifeng Zhang, Yu Yang, Min
Description	Author Affiliation: Liu R ( College of Environmental and Resource Sciences, University of Chinese Academy of Sciences, Beijing 100049, China); Gao Y ( Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China E-mail: yingxingao@sina.com.); Ji Y ( Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China E-mail: yingxingao@sina.com); Zhang Y ( Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China E-mail: yingxingao@sina.com.); Yang M ( Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China E-mail: yingxingao@sina.com.)
Abstract	Alkane and aromatic hydroxylase genes in a full-scale aerobic system treating oily produced wastewater under thermophilic condition (45-50 °C) in the Jidong oilfield, China, were investigated using clone library and quantitative polymerase chain reaction methods. Rather than the normally encountered integral-membrane non-haem iron monooxygenase (alkB) genes, only CYP153-type P450 hydroxylase genes were detected for the alkane activation, indicating that the terminal oxidation of alkanes might be mainly mediated by the CYP153-type alkane hydroxylases in the thermophilic aerobic process. Most of the obtained CYP153 gene clones showed distant homology with the reference sequences, which might represent novel alkane hydroxylases. For the aromatic activation, the polycyclic aromatic hydrocarbon-ring hydroxylating dioxygenase (PAH-RHD) gene was derived from Gram-negative PAH-degraders belonging to the Burkholderiales order, with a 0.72% relative abundance of PAH-RHD gene to 16S rRNA gene. This was consistent with the result of 16S rRNA gene analysis, indicating that Burkholderiales bacteria might play a key role in the full-scale process of thermophilic hydrocarbon degradation.
File Format	HTM / HTML
ISSN	02731223
Issue Number	1
Volume Number	71
e-ISSN	19969732
Journal	Water Science & Technology
Language	English
Publisher	IWA Publishing
Publisher Date	2015-01-01
Publisher Place	Great Britain (UK)
Access Restriction	Subscribed
Subject Keyword	Discipline Hydrology Bacterial Proteins Genetics Betaproteobacteria Enzymology Cytochrome P-450 Cyp4a Dioxygenases Gram-negative Aerobic Rods And Cocci Aerobiosis Metabolism Bioreactors Microbiology Hot Temperature Mixed Function Oxygenases Molecular Sequence Data Phylogeny Rna, Ribosomal, 16s Sequence Analysis, Dna Waste Disposal, Fluid Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Environmental Engineering Water Science and Technology

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