NDLI: Interleukin-11 receptor is overexpressed in human osteosarcoma, and near-infrared-labeled IL-11R imaging agent could detect osteosarcoma in mouse tumor xenografts.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liu, Tao Ma, Qiong Zhang, Yinglong Ke, Shi Yan, Kang Chen, Xiang Wen, Yanhua Fan, Qingyu Qiu, Xiuchun
Description	Author Affiliation: Liu T ( Department of Orthopedic Surgery, Tangdu Hospital, Fourth Military Medical University, Xi'an, Shanxi, 710038, People's Republic of China.)
Abstract	IL-11R is an important cytokine receptor that links oxidative stress and compensatory proliferation. Mounting evidence has demonstrated that IL-11R regulates autoimmune demyelination and the invasion and proliferation of cancer cells, making it an important therapeutic target for molecular targeted therapy. Moreover, overexpression of IL-11R indicates a poor long-term prognosis in cancer patients. However, the expression status and its potential as a biomarker for diagnosis, tumor imaging, and prognosis in osteosarcoma remain to be determined. We report here that IL-11R is highly expressed in osteosarcoma and near-infrared (NIR)-labeled IL-11R imaging agent could detect osteosarcoma in mouse tumor xenografts. In a panel of human osteosarcoma specimens, IL-11R protein was positively stained in most cases by immunohistochemistry. Western blot analysis and flow cytometry showed that IL-11R was overexpressed in osteosarcoma SOSP-9607 cells. Cell-binding assay demonstrated specific binding of the IL-11R targeted imaging agent to osteosarcoma SOSP-9607 cells in vitro. In addition, administration of an IL-11R targeted imaging agent in a nude mice orthotopic model resulted in selective accumulation of NIR fluorescent signals in the bone tumor as well as several metabolic organs. These results indicate that IL-11R is a potential target for the development of molecular targeted therapy and noninvasive tumor imaging in human osteosarcoma. Furthermore, NIR-labeled IL-11R imaging agent is a promising lead for the development of a tumor in vivo imaging method at the molecular level in the management of human osteosarcoma.
File Format	HTM / HTML
ISSN	10104283
Issue Number	4
Volume Number	36
e-ISSN	14230380
Journal	Tumor Biology
Language	English
Publisher	Springer
Publisher Date	2015-04-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Medicine__semicolon__oncology Bone Neoplasms Genetics Interleukin-11 Receptor Alpha Subunit Biosynthesis Molecular Imaging Osteosarcoma Animals Pathology Cell Line, Tumor Cell Tracking Flow Cytometry Gene Expression Regulation, Neoplastic Humans Mice Molecular Targeted Therapy Xenograft Model Antitumor Assays Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in