NDLI: RETRACTED ARTICLE: MiR-34a suppresses ovarian cancer proliferation and motility by targeting AXL

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Li, Rui Shi, Xuejun Ling, Fengyu Wang, Chunguang Liu, Junxia Wang, Wei Li, Ming
Description	Country affiliation: China Author Affiliation: Li R ( Department of Oncology, Yongchuan Hospital Affiliated to Chongqing Medical University, No. 439, Xuanhua Road, Chongqing, 402160, China.); Shi X ( Department of Oncology, Yongchuan Hospital Affiliated to Chongqing Medical University, No. 439, Xuanhua Road, Chongqing, 402160, China. drshixj6@163.com.); Ling F ( Department of Oncology, Yongchuan Hospital Affiliated to Chongqing Medical University, No. 439, Xuanhua Road, Chongqing, 402160, China.); Wang C ( Department of Oncology, Yongchuan Hospital Affiliated to Chongqing Medical University, No. 439, Xuanhua Road, Chongqing, 402160, China.); Liu J ( Department of Oncology, Yongchuan Hospital Affiliated to Chongqing Medical University, No. 439, Xuanhua Road, Chongqing, 402160, China.); Wang W ( Department of Oncology, Yongchuan Hospital Affiliated to Chongqing Medical University, No. 439, Xuanhua Road, Chongqing, 402160, China.); Li M ( Department of Oncology, Yongchuan Hospital Affiliated to Chongqing Medical University, No. 439, Xuanhua Road, Chongqing, 402160, China.)
Abstract	Increasing evidence has suggested that dysregulation of microRNAs (miRNAs) could contribute to tumor progression. The miR-34 family is directly transactivated by tumor suppressor p53 which is frequently mutated in various cancers; however, the effect of miR-34a on the ovarian cancer cells remains unclear. The aim of the paper was to study the expression of miR-34a in ovarian cancer and miR-34a’s relation to the cell proliferation and metastasis in ovarian cancer in vitro. miR-34a expression was determined by quantitative RT-PCR in a panel of 60 human ovarian cancer samples. Functional characterization of miR-34a was accomplished by reconstitution of miR-34a expression in ovarian cancer cells by determining changes in proliferation, migration, and invasion. Our results showed that miR-34a is downregulated in ovarian cancer tissues compared with the corresponding adjacent non-neoplastic tissues, and the expression level of miR-34a was significantly lower in ovarian cancer cell lines in comparison with normal human fallopian tube epithelial cell line. The 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-diphenytetrazoliumromide (MTT) assay revealed significant cell proliferation inhibition in miR-34a transfectant compared with the control from HO8910 and SKOV3 cells, which displayed lowest expressions of miR-34a. Furthermore, the transwell assay also showed significant cell migration inhibition in miR-34a transfectant, compared with cell lines transfected with NC. Overexpression of miR-34a led to the inhibition of AXL expression, indicating that AXL is a target gene for miR-34a. Our data suggest that miR-34a may function as a tumor suppressor through repression of oncogenic AXL in ovarian cancer.
File Format	HTM / HTML
ISSN	10104283
Issue Number	9
Volume Number	36
e-ISSN	14230380
Journal	Tumor Biology
Language	English
Publisher	Springer
Publisher Date	2015-09-01
Publisher Place	Netherlands
Access Restriction	Subscribed
Subject Keyword	Discipline Medicine__semicolon__oncology Micrornas Genetics Ovarian Neoplasms Proto-oncogene Proteins Receptor Protein-tyrosine Kinases Tumor Suppressor Protein P53 Apoptosis Cell Line, Tumor Cell Movement Cell Proliferation Fallopian Tubes Cytology Metabolism Female Gene Expression Regulation, Neoplastic Humans Biosynthesis Neoplasm Invasiveness Neoplasm Metastasis Pathology Antagonists & Inhibitors Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Cancer Research

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