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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | de la Cruz-Vicente, F. Perez-Romero, P. Aguilar-Guisado, M. Cisneros-Herreros, J. M. Urbano-Ispizua, A. Espigado, I. |
| Description | Country affiliation: Spain Author Affiliation: de la Cruz-Vicente F ( Hematology and Hemotherapy Department, Spain Hospital Universitario Virgen del Rocio, Seville, Spain. fatimadelacruzv@gmail.com) |
| Abstract | Fifty percent of allogeneic stem cell recipients develop cytomegalovirus (CMV) infection in the first 100 days posttransplantation. Various methods have been used to determine CMV infections, including antigenemia assay and real-time polymerase chain reaction (RT-PCR). Although antigenemia assay has been used more frequently, this technique is less sensitive than RT-PCR. In contrast, RT-PCR has a low positive predictive value for CMV end-organ disease. Cytomegalovirus infections were analyzed in 41 peripheral blood samples from allogeneic stem cell recipients using both antigenemia assay and RT-PCR; results were discordant in 36.6% of patients. Although the antigenemia assay detected CMV replication in 29.2% of cases, RT-PCR was positive in 65.8%. In 83.3% of patients, results detected using the antigenemia assay were delayed by a median (range) of 5 (2-20) weeks compared with positive RT-PCR results. Within the first 100 days posttransplantation, higher levels of viral replication measured using RT-PCR were observed in patients with vs without antigenemia. In addition, in patients with antigenemia, viral load was significantly higher before day 100 than after (P=.01 and P=.008, respectively) compared with those without antigenemia. |
| File Format | HTM / HTML |
| ISSN | 00411345 |
| Issue Number | 8 |
| Volume Number | 42 |
| e-ISSN | 18732623 |
| Journal | Transplantation Proceedings |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-10-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Transplantation__semicolon__medicine Antigens, Viral Blood Cytomegalovirus Physiology Polymerase Chain Reaction Methods Stem Cell Transplantation Virus Replication Genetics Immunology Humans Retrospective Studies Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Transplantation Surgery |
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