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Metabolic profile of pancreatic acinar and islet tissue in culture.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Suszynski, T. M. Mueller, K. R. Gruessner, A. C. Papas, K. K.
Description	Country affiliation: Mongolia Author Affiliation: Suszynski TM ( Department of Surgery, University of Minnesota, Minneapolis, MN.); Mueller KR ( Department of Surgery, University of Minnesota, Minneapolis, MN.); Gruessner AC ( Institute for Cellular Transplantation, University of Arizona, Tucson, AZ 85724.); Papas KK ( Institute for Cellular Transplantation, University of Arizona, Tucson, AZ 85724. Electronic address: kkpapas@email.arizona.edu.)
Abstract	BACKGROUND: The amount and condition of exocrine impurities may affect the quality of islet preparations, especially during culture. In this study, the objective was to determine the oxygen demand and viability of islet and acinar tissue post-isolation and whether they change disproportionately while in culture. METHOD: We compared the oxygen consumption rate (OCR) normalized to DNA (OCR/DNA, a measure of fractional viability in units of nmol/min/mg DNA), and the percent change in OCR and DNA recoveries between adult porcine islet and acinar tissue from the same preparation (paired) over 6-9 days of standard culture. Paired comparisons were done to quantify differences in OCR/DNA between islet and acinar tissue from the same preparation, at specified time points during culture. RESULTS: The mean (±SE) OCR/DNA was 74.0 ± 11.7 units higher for acinar (vs islet) tissue on the day of isolation (n = 16, P < .0001), but 25.7 ± 9.4 units lower after 1 day (n = 8, P = .03), 56.6 ± 11.5 units lower after 2 days (n = 12, P = .0004), and 65.9 ± 28.7 units lower after 8 days (n = 4, P = .2) in culture. DNA and OCR recoveries decreased at different rates for acinar versus islet tissue over 6-9 days in culture (n = 6). DNA recovery decreased to 24 ± 7% for acinar and 75 ± 8% for islets (P = .002). Similarly, OCR recovery decreased to 16 ± 3% for acinar and remained virtually constant for islets (P = .005). CONCLUSION: Differences in the metabolic profile of acinar and islet tissue should be considered when culturing impure islet preparations. OCR-based measurements may help optimize pre-islet transplantation culture protocols.
File Format	HTM / HTML
ISSN	00411345
e-ISSN	18732623
DOI	10.1016/j.transproceed.2014.06.003
Journal	Transplantation Proceedings
Issue Number	6
Volume Number	46
Language	English
Publisher	Elsevier
Publisher Date	2014-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Transplantation__semicolon__medicine Islets Of Langerhans Metabolism Metabolome Physiology Oxygen Consumption Pancreas, Exocrine Animals Islets Of Langerhans Transplantation Swine Time Factors Tissue Culture Techniques Tissue Survival Tissue And Organ Harvesting Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Transplantation Surgery

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A good breath of oxygen for beta-like cells obtained from porcine exocrine pancreatic tissue.

Improved method of porcine pancreas procurement with arterial flush and ductal injection enhances islet isolation outcome.

Cryo-isolation: a novel method for enzyme-free isolation of pancreatic islets involving in situ cryopreservation of islets and selective destruction of acinar tissue.

Human islet isolation processing times shortened by one hour: minimized incubation time between tissue harvest and islet purification.

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A new oxygen carrier for improved long-term storage of human pancreata before islet isolation.

Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance.

Islet Preparation Purity Is Overestimated, and Less Pure Fractions Have Lower Post-Culture Viability Before Clinical Allotransplantation

Metabolic profile of pancreatic acinar and islet tissue in culture.

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A good breath of oxygen for beta-like cells obtained from porcine exocrine pancreatic tissue.

Improved method of porcine pancreas procurement with arterial flush and ductal injection enhances islet isolation outcome.

Cryo-isolation: a novel method for enzyme-free isolation of pancreatic islets involving in situ cryopreservation of islets and selective destruction of acinar tissue.

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Assessment of tissue-engineered islet graft viability by fluorine magnetic resonance spectroscopy.

A new oxygen carrier for improved long-term storage of human pancreata before islet isolation.

Pancreas oxygen persufflation increases ATP levels as shown by nuclear magnetic resonance.

Islet Preparation Purity Is Overestimated, and Less Pure Fractions Have Lower Post-Culture Viability Before Clinical Allotransplantation

Metabolic profile of pancreatic acinar and islet tissue in culture.