NDLI: Urinary Peptide patterns in native kidneys and kidney allografts.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhang, Yan Oetting, William S. Harvey, Stephen B. Stone, Matthew D. Monkkonen, Teresa Matas, Arthur J. Cosio, Fernando G. Nelsestuen, Gary L.
Description	Country affiliation: United States Author Affiliation: Zhang Y ( Department of Biochemistry, Molecular Biology, and Biophysics, University of Minnesota, Minneapolis, MN 55455, USA.)
Abstract	BACKGROUND: The use of biopsies to determine kidney health after kidney transplantation is an invasive procedure with some risk to the patient. Consequently, a noninvasive test for transplanted kidney health would provide a significant advantage over current clinical practice. METHODS: Urines from kidney donors before nephrectomy, pretransplant patients with native kidney disease, and posttransplant kidney recipients were examined for protein biomarkers to diagnose or prognose kidney disease. Proteins were extracted by C4 reverse phase affinity and analyzed by matrix assisted laser desorption/ionization time-of-flight mass spectrometry. RESULTS: Urine from individuals with healthy kidneys showed few components other than two ubiquitous saposin B glycoisoforms. Patients with kidney disease lacked saposin B and showed new components in two patterns: the most common contained beta-2 microglobulin (B2M, m/z=11,732) plus one or more peaks at m/z=10,350, 9480, 4337, and 4180. Pattern 2 lacked beta-2 microglobulin but contained several degradation products of alpha-1 antitrypsin. Other pathologic components included urinary protein 1 (m/z=15,835), transthyretin (m/z=13,880), and a component at m/z=13,350. CONCLUSIONS: Patients with acute rejection showed profiles that ranged from those of kidney donors to those of advanced kidney disease. The range of patterns may be useful for analysis of transplant patients without complications and persons with developing kidney disease before or after transplant.
File Format	HTM / HTML
ISSN	00411337
e-ISSN	15346080
DOI	10.1097/TP.0b013e3181a66595
Journal	Transplantation Journal
Issue Number	12
Volume Number	87
Language	English
Publisher	Lippincott Williams & Wilkins
Publisher Date	2009-06-27
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Transplantation Kidney Transplantation Physiology Kidney Peptides Urine Adolescent Chromatography, Affinity Diabetes Mellitus Cytology Pathology Nephrectomy Isolation & Purification Polycystic Kidney Diseases Reference Values Saposins Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Transplantation, Homologous Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Transplantation

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