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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | de Carvalho Niebel, F. Frendo, P. Van Montagu, M. Cornelissen, M. |
| Description | Country affiliation: Belgium Author Affiliation: de Carvalho Niebel F ( Laboratorium voor Genetica, Universiteit Gent, Belgium.) |
| Abstract | Silencing of a Nicotiana plumbaginifolia beta-1,3-glucanase (gn1) transgene in tobacco line T17 occurs in homozygous and in haploid plants with one transgene locus dosage per chromosome set. We have previously shown that the silent state is manifested by a reduced gn1 steady state mRNA level and results from a post-transcriptional process that is under developmental control in homozygous T17 plants. In this study, we show that specific endogenous beta-1,3-glucanase genes are cosuppressed with gn1 in homozygous T17 plants. We also demonstrate that the developmental timing of cosuppression depends on environmental conditions and that once silencing is established it is stably maintained during plant development. Analyses of additional transgenic tobacco lines revealed that gn1 silencing is not restricted to the T17 line and showed that silencing can also take place in R0 plants containing independent loci in hemizygous states. Furthermore, silencing can also be obtained in progeny plants in which expressing loci have been combined. Importantly, cosuppression occurs via a post-transcriptional mechanism that does not interfere with the accumulation of transgene nuclear mRNA. These results strongly suggest that the silencing mechanism operates at RNA transport and/or RNA stability levels. |
| File Format | HTM / HTML |
| ISSN | 10404651 |
| e-ISSN | 1531298X |
| Journal | THE PLANT CELL ONLINE |
| Issue Number | 3 |
| Volume Number | 7 |
| Language | English |
| Publisher | American Society of Plant Biologists |
| Publisher Date | 1995-03-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Botany Cell Nucleus Metabolism Rna Processing, Post-transcriptional Rna, Messenger Suppression, Genetic Beta-glucosidase Biosynthesis Dna Primers Gene Expression Regulation, Plant Glucan 1,3-beta-glucosidase Homozygote Molecular Sequence Data Plants, Genetically Modified Plants, Toxic Polymerase Chain Reaction Tobacco Enzymology Genetics Transcription, Genetic Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Plant Science |
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