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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ulmasov, T. Liu, Z. B. Hagen, G. Guilfoyle, T. J. |
| Description | Country affiliation: United States Author Affiliation: Ulmasov T ( Department of Biochemistry, University of Missouri, Columbia 65211, USA.) |
| Abstract | The auxin-responsive soybean GH3 gene promoter is composed of multiple auxin response elements (AuxREs), and each AuxRE contributes incrementally to the strong auxin inducibility to the promoter. Two independent AuxREs of 25 bp (D1) and 32 bp (D4) contain the sequence TGTCTC. Results presented here show that the TGTCTC element in D1 and D4 is required but not sufficient for auxin inducibility in carrot protoplast transient expression assays. Additional nucleotides upstream of TGTCTC are also required for auxin inducibility. These upstream sequences showed constitutive activity and no auxin inducibility when part or all of the TGTCTC element was mutated or deleted. In D1, the constitutive element overlaps the 5' portion of TGTCTC; in D4, the constitutive element is separated from TGTCTC. An 11-bp element in D1, CCTCGTGTCTC, conferred auxin inducibility to a minimal cauliflower mosaic virus 35S promoter in transgenic tobacco seedlings as well as in carrot protoplasts (i.e., transient expression assays). Both constitutive elements bound specifically to plant nuclear proteins, and the constitutive element in D1 bound to a recombinant soybean basic leucine zipper transcription factor with G-box specificity. To demonstrate further the composite nature of AuxREs and the ability of the TGTCTC element to confer auxin inducibility, we created a novel AuxRE by placing a yeast GAL4 DNA binding site adjacent to the TGTCTC element. Expression of a GAL4-c-Rel transactivator in the presence of this novel AuxRE resulted in auxin-inducible expression. Our results indicate that at least some AuxREs have a composite structure consisting of a constitutive element adjacent to a conserved TGTCTC element that confers auxin inducibility. |
| File Format | HTM / HTML |
| ISSN | 10404651 |
| e-ISSN | 1531298X |
| Journal | THE PLANT CELL ONLINE |
| Issue Number | 10 |
| Volume Number | 7 |
| Language | English |
| Publisher | American Society of Plant Biologists |
| Publisher Date | 1995-10-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Botany Genes, Plant Drug Effects Indoleacetic Acids Pharmacology Animals Dna, Plant Genetics Metabolism Molecular Sequence Data Mutation Nuclear Proteins Plant Proteins Plants, Genetically Modified Plants, Toxic Promoter Regions, Genetic Soybeans Tobacco Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Plant Science |
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