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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Albani, D. Hammond-Kosack, M. C. Smith, C. Conlan, S. Colot, V. Holdsworth, M. Bevan, M. W. |
| Description | Country affiliation: United kingdom Author Affiliation: Albani D ( Molecular Genetics Department, John Innes Centre, Norwich, United Kingdom.) |
| Abstract | The conserved bifactorial endosperm box found in the promoter of wheat storage protein genes comprises two different cis elements that are thought to be involved in regulating endosperm-specific gene expression. Endosperm nuclear extracts contain binding activities. One is called ESBF-I, which binds to the endosperm motif (EM), and the other is called ESBF-II, which binds to the GCN4-like motif(GLM). Here, we present a functional analysis of the endosperm box of a low-molecular-weight glutenin gene found on the 1D1 chromosome of hexaploid wheat (LMWG-1D1) in transgenic tobacco plants. Our analysis demonstrates the necessity of the EM and GLM for endosperm-specific gene expression and suggests the presence in tobacco of functional counterparts of wheat ESBF-I and ESBF-II. Furthermore, we describe the isolation and characterization of cDNA clones encoding SPA, a seed-specific basic leucine zipper protein from wheat that can activate transcription from the GLMs of the -326-bp LMWG-1D1 promoter in both maize and tobacco leaf protoplasts. This activation is also partially dependent on the presence of functional EMs, suggesting interactions between SPA with ESBF-I-like activities. |
| File Format | HTM / HTML |
| ISSN | 10404651 |
| e-ISSN | 1531298X |
| Journal | THE PLANT CELL ONLINE |
| Issue Number | 2 |
| Volume Number | 9 |
| Language | English |
| Publisher | American Society of Plant Biologists |
| Publisher Date | 1997-02-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Botany Dna-binding Proteins Metabolism Fungal Proteins Leucine Zippers Plant Proteins Genetics Protein Kinases Saccharomyces Cerevisiae Proteins Trans-activators Transcription Factors Amino Acid Sequence Basic-leucine Zipper Transcription Factors G-box Binding Factors Molecular Sequence Data Plants, Genetically Modified Plants, Toxic Prolamins Promoter Regions, Genetic Tobacco Triticum Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Plant Science |
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