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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Batoko, H. Zheng, H. Q. Hawes, C. Moore, I. |
| Description | Country affiliation: United kingdom Author Affiliation: Batoko H ( Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom.) |
| Abstract | We describe a green fluorescent protein (GFP)-based assay for investigating membrane traffic on the secretory pathway in plants. Expression of AtRab1b(N121I), predicted to be a dominant inhibitory mutant of the Arabidopsis Rab GTPase AtRab1b, resulted in accumulation of a secreted GFP marker in an intracellular reticulate compartment reminiscent of the endoplasmic reticulum. This accumulation was alleviated by coexpressing wild-type AtRab1b but not AtRab8c. When a Golgi-targeted and N-glycosylated variant of GFP was coexpressed with AtRab1b(N121I), the variant also accumulated in a reticulate network and an endoglycosidase H-sensitive population appeared. Unexpectedly, expression of AtRab1b(N121I), but not of the wild-type AtRab1b, resulted in a reduction or cessation of vectorial Golgi movement, an effect that was reversed by coexpression of the wild type. We conclude that AtRab1b function is required for transport from the endoplasmic reticulum to the Golgi apparatus and suggest that this process may be coupled to the control of Golgi movement. |
| File Format | HTM / HTML |
| ISSN | 10404651 |
| e-ISSN | 1531298X |
| Journal | THE PLANT CELL ONLINE |
| Issue Number | 11 |
| Volume Number | 12 |
| Language | English |
| Publisher | American Society of Plant Biologists |
| Publisher Date | 2000-11-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Botany Arabidopsis Metabolism Endoplasmic Reticulum Gtp Phosphohydrolases Golgi Apparatus Plants, Toxic Tobacco Rab1 Gtp-binding Proteins Enzymology Dna Primers Fluorescence Glycoside Hydrolases Glycosylation Green Fluorescent Proteins Hydrolysis Luminescent Proteins Protein Transport Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Plant Science |
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