NDLI: The Catalytically Active Tyrosine Residues of Both SPO11-1 and SPO11-2 Are Required for Meiotic Double-Strand Break Induction in Arabidopsis

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The Catalytically Active Tyrosine Residues of Both SPO11-1 and SPO11-2 Are Required for Meiotic Double-Strand Break Induction in Arabidopsis

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Hartung, Frank Wurz-Wildersinn, Rebecca Fuchs, Jörg Schubert, Ingo Suer, Stefanie Puchta, Holger
Description	Country affiliation: Germany Author Affiliation: Hartung F ( Botanisches Institut II, Universität Karlsruhe, 76128 Karlsruhe, Germany.)
Abstract	SPO11, a homolog of the subunit A of the archaebacterial topoisomerase VI, is essential for double-strand break (DSB)-induced initiation of meiotic recombination. In contrast with single homologs in animals and yeasts, three homologs are present in Arabidopsis thaliana and other higher plants. Whereas At SPO11-3 is involved in somatic endoreduplication, At SPO11-1 and, as recently shown, At SPO11-2 are essential for the initiation of meiotic recombination. Further defining the role of At SPO11-2, we were able to demonstrate that it is required for proper chromosome segregation, as its loss resulted in aneuploidy in the surviving progeny. The double mutant spo11-1 spo11-2 does not differ phenotypically from the single mutants, indicating that both proteins are required for the same step. Contrary to the observations for the At rad51-1 single mutant, the combination of spo11-2 and rad51-1 did not lead to chromosome fragmentation, indicating that SPO11-2, like SPO11-1, is required for DSB induction. As the meiotic phenotype of both single SPO11 mutants can be reversed by complementation using the full-length genes but not the same constructs mutated in their respective catalytically active Tyr, both proteins seem to participate directly in the DNA breakage reaction. The active involvement of two SPO11 homologs for DSB formation reveals a striking difference between plants and other eukaryotes in meiosis.
File Format	HTM / HTML
ISSN	10404651
e-ISSN	1531298X
Journal	THE PLANT CELL ONLINE
Issue Number	10
Volume Number	19
Language	English
Publisher	American Society of Plant Biologists
Publisher Date	2007-10-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Botany Arabidopsis Proteins Metabolism Arabidopsis Dna Breaks, Double-stranded Dna Topoisomerases Tyrosine Genetics Chromosome Pairing Chromosome Segregation Flow Cytometry Genetic Complementation Test In Situ Hybridization, Fluorescence Meiosis Models, Genetic Mutation Phenotype Reproduction Reverse Transcriptase Polymerase Chain Reaction Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science

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The Catalytically Active Tyrosine Residues of Both SPO11-1 and SPO11-2 Are Required for Meiotic Double-Strand Break Induction in Arabidopsis

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The Catalytically Active Tyrosine Residues of Both SPO11-1 and SPO11-2 Are Required for Meiotic Double-Strand Break Induction in Arabidopsis

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The Catalytically Active Tyrosine Residues of Both SPO11-1 and SPO11-2 Are Required for Meiotic Double-Strand Break Induction in Arabidopsis