NDLI: Role of aminoalcoholphosphotransferases 1 and 2 in phospholipid homeostasis in Arabidopsis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liu, Yu Wang, Geliang Wang, Xuemin
Description	Author Affiliation: Liu Y ( Department of Biology, University of Missouri, St. Louis, Missouri 63121 Donald Danforth Plant Science Center, St. Louis, Missouri 63132.); Wang G ( Department of Biology, University of Missouri, St. Louis, Missouri 63121 Donald Danforth Plant Science Center, St. Louis, Missouri 63132.); Wang X ( Department of Biology, University of Missouri, St. Louis, Missouri 63121 Donald Danforth Plant Science Center, St. Louis, Missouri 63132 swang@danforthcenter.org.)
Abstract	Aminoalcoholphosphotransferase (AAPT) catalyzes the synthesis of phosphatidylcholine (PC) and phosphotidylethanolamine (PE), which are the most prevalent membrane phospholipids in all eukaryotic cells. Here, we show that suppression of AAPTs results in extensive membrane phospholipid remodeling in Arabidopsis thaliana. Double knockout (KO) mutants that are hemizygous for either aapt1 or aapt2 display impaired pollen and seed development, leading to embryotic lethality of the double KO plants, whereas aapt1 or aapt2 single KO plants show no overt phenotypic alterations. The growth rate and seed yield of AAPT RNA interference (RNAi) plants are greatly reduced. Lipid profiling shows decreased total galactolipid and phospholipid content in aapt1-containing mutants, including aapt1, aapt1/aapt1 aapt2/AAPT2, aapt1/AAPT1 aapt2/aapt2, and AAPT RNAi plants. The level of PC in leaves was unchanged, whereas that of PE was reduced in all AAPT-deficient plants, except aapt2 KO. However, the acyl species of PC was altered, with increased levels of C34 species and decreased C36 species. Conversely, the levels of PE and phosphatidylinositol were decreased in C34 species. In seeds, all AAPT-deficient plants, including aapt2 KO, displayed a decrease in PE. The data show that AAPT1 and AAPT2 are essential to plant vegetative growth and reproduction and have overlapping functions but that AAPT1 contributes more than AAPT2 to PC production in vegetative tissues. The opposite changes in molecular species between PC and PE and unchanged PC level indicate the existence of additional pathways that maintain homeostatic levels of PC, which are crucial for the survival and proper development of plants.
File Format	HTM / HTML
ISSN	10404651
e-ISSN	1531298X
DOI	10.1105/tpc.15.00180
Journal	THE PLANT CELL ONLINE
Issue Number	5
Volume Number	27
Language	English
Publisher	American Society of Plant Biologists
Publisher Date	2015-05-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Botany Arabidopsis Enzymology Gene Expression Regulation, Plant Phospholipids Metabolism Phosphotransferases Genetics Growth & Development Physiology Arabidopsis Proteins Homeostasis Mutation Phosphatidylcholines Plants, Genetically Modified Pollen Reproduction Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Cell Biology Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Downy mildew resistance in Arabidopsis by mutation of HOMOSERINE KINASE.

Gibberellins are required for seed development and pollen tube growth in Arabidopsis.

Dual role for phospholipid:diacylglycerol acyltransferase: enhancing fatty acid synthesis and diverting fatty acids from membrane lipids to triacylglycerol in Arabidopsis leaves.

Regulation of axis determinacy by the Arabidopsis PINHEAD gene.

ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 are Polygalacturonases required for cell separation during reproductive development in Arabidopsis.

Endoplasmic reticulum- and Golgi-localized phospholipase A2 plays critical roles in Arabidopsis pollen development and germination.

AtOPT3, a member of the oligopeptide transporter family, is essential for embryo development in Arabidopsis.

Microtubule-associated proteins MAP65-1 and MAP65-2 positively regulate axial cell growth in etiolated Arabidopsis hypocotyls.

Plasma membrane-associated ROP10 small GTPase is a specific negative regulator of abscisic acid responses in Arabidopsis.

Role of aminoalcoholphosphotransferases 1 and 2 in phospholipid homeostasis in Arabidopsis.

Similar Documents

Downy mildew resistance in Arabidopsis by mutation of HOMOSERINE KINASE.

Gibberellins are required for seed development and pollen tube growth in Arabidopsis.

Dual role for phospholipid:diacylglycerol acyltransferase: enhancing fatty acid synthesis and diverting fatty acids from membrane lipids to triacylglycerol in Arabidopsis leaves.

Regulation of axis determinacy by the Arabidopsis PINHEAD gene.

ARABIDOPSIS DEHISCENCE ZONE POLYGALACTURONASE1 (ADPG1), ADPG2, and QUARTET2 are Polygalacturonases required for cell separation during reproductive development in Arabidopsis.

Endoplasmic reticulum- and Golgi-localized phospholipase A2 plays critical roles in Arabidopsis pollen development and germination.

AtOPT3, a member of the oligopeptide transporter family, is essential for embryo development in Arabidopsis.

Microtubule-associated proteins MAP65-1 and MAP65-2 positively regulate axial cell growth in etiolated Arabidopsis hypocotyls.

Plasma membrane-associated ROP10 small GTPase is a specific negative regulator of abscisic acid responses in Arabidopsis.

Role of aminoalcoholphosphotransferases 1 and 2 in phospholipid homeostasis in Arabidopsis.