NDLI: Cancer Cell Phenotype Dependent Differential Intracellular Trafficking of Unconjugated Quantum Dots

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Barua, Sutapa Rege, Kaushal
Description	Country affiliation: United States Author Affiliation: Barua S ( Department of Chemical Engineering, Arizona State University, Tempe, AZ 85287-6006, USA.)
Abstract	A diverse array of nanoparticles, including quantum dots (QDs), metals, polymers, liposomes, and dendrimers, are being investigated as therapeutics and imaging agents in cancer diseases. However, the role of the cancer-cell phenotype on the uptake and intracellular fate of nanoparticles in cancer cells remains poorly understood. Reported here is that differences in cancer-cell phenotypes can lead to significant differences in intracellular sorting, trafficking, and localization of nanoparticles. Unconjugated anionic QDs demonstrate dramatically different intracellular profiles in three closely related human-prostate-cancer cells used in the investigation: PC3, PC3-flu, and PC3-PSMA. QDs demonstrate punctated intracellular localization throughout the cytoplasm in PC3 cells. In contrast, the nanoparticles localize mainly at a single juxtanuclear location ('dot-of-dots') inside the perinuclear recycling compartment in PC3-PSMA cells, where they co-localize with transferrin and the prostate-specific membrane antigen. The results indicate that nanoparticle sorting and transport is influenced by changes in cancer-cell phenotype and can have significant implications in the design and engineering of nanoscale drug delivery and imaging systems for advanced tumors.
File Format	HTM / HTML
ISSN	16136810
e-ISSN	16136829
DOI	10.1002/smll.200800972
Journal	Small
Issue Number	3
Volume Number	5
Language	English
Publisher	Wiley-VCH
Publisher Date	2009-03-01
Publisher Place	Germany
Access Restriction	Open
Subject Keyword	Discipline Nanotechnology Prostatic Neoplasms Metabolism Quantum Dots Biological Transport Cell Line, Tumor Clathrin Drug Delivery Systems Microscopy, Confocal Microscopy, Fluorescence Phenotype Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Chemistry Nanoscience and Nanotechnology Medicine Biomaterials Engineering Biotechnology

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Cancer Cell Phenotype Dependent Differential Intracellular Trafficking of Unconjugated Quantum Dots

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Cancer Cell Phenotype Dependent Differential Intracellular Trafficking of Unconjugated Quantum Dots

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Cancer Cell Phenotype Dependent Differential Intracellular Trafficking of Unconjugated Quantum Dots