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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Aaron, Jesse S. Greene, Adrienne C. Kotula, Paul G. Bachand, George D. Timlin, Jerilyn A. |
| Description | Country affiliation: United States Author Affiliation: Aaron JS ( Sandia National Laboratories, PO Box 5800, MS-0895, Albuquerque, NM 87185, USA.) |
| Abstract | The biocompatibility and possible toxicological consequences of engineered nanomaterials, including quantum dots (QDs) due to their unique suitability for biomedical applications, remain intense areas of interest. We utilized advanced imaging approaches to characterize the interactions of CdSe QDs of various sizes and shapes with live immune cells. Particle diffusion and partitioning within the plasma membrane, cellular uptake kinetics, and sorting of particles into lysosomes were all independantly characterized. Using high-speed total internal reflectance fluorescence (TIRF) microscopy, we show that QDs with an average aspect ratio of 2.0 (i.e., rod-shaped) diffuse nearly an order of magnitude slower in the plasma membrane than more spherical particles with aspect ratios of 1.2 and 1.6, respectively. Moreover, more rod-shaped QDs were shown to be internalized into the cell 2-3 fold more slowly. Hyperspectral confocal fluorescence microscopy demonstrates that QDs tend to partition within the cell membrane into regions containing a single particle type. Furthermore, data examining QD sorting mechanisms indicate that endocytosis and lysosomal sorting increases with particle size. Together, these observations suggest that both size and aspect ratio of a nanoparticle are important characteristics that significantly impact interactions with the plasma membrane, uptake into the cell, and localization within intracellular vesicles. Thus, rather than simply characterizing nanoparticle uptake into cells, we show that utilization of advanced imaging approaches permits a more nuanced and complete examination of the multiple aspects of cell-nanoparticle interactions that can ultimately aid understanding possible mechanisms of toxicity, resulting in safer nanomaterial designs. |
| File Format | HTM / HTML |
| ISSN | 16136810 |
| Issue Number | 3 |
| Volume Number | 7 |
| e-ISSN | 16136829 |
| Journal | Small |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2011-02-07 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Nanotechnology Nanotechnology Methods Quantum Dots Cadmium Compounds Chemistry Microscopy, Confocal Microscopy, Electron, Scanning Transmission Nanoparticles Ultrastructure Selenium Compounds Journal Article Research Support, U.s. Gov't, Non-p.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Chemistry Nanoscience and Nanotechnology Medicine Biomaterials Engineering Biotechnology |
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