NDLI: Medicago truncatula DNF2 is a PI-PLC-XD-containing protein required for bacteroid persistence and prevention of nodule early senescence and defense-like reactions.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bourcy, Marie Brocard, Lysiane Pislariu, Catalina I. Cosson, Viviane Mergaert, Peter Tadege, Millon Mysore, Kirankumar S. Udvardi, Michael K. Gourion, Benjamin Ratet, Pascal
Description	Country affiliation: France Author Affiliation: Bourcy M ( Institut des Sciences du Végétal, CNRS, Gif Sur Yvette, France.)
Abstract	Medicago truncatula and Sinorhizobium meliloti form a symbiotic association resulting in the formation of nitrogen-fixing nodules. Nodule cells contain large numbers of bacteroids which are differentiated, nitrogen-fixing forms of the symbiotic bacteria. In the nodules, symbiotic plant cells home and maintain hundreds of viable bacteria. In order to better understand the molecular mechanism sustaining the phenomenon, we searched for new plant genes required for effective symbiosis. We used a combination of forward and reverse genetics approaches to identify a gene required for nitrogen fixation, and we used cell and molecular biology to characterize the mutant phenotype and to gain an insight into gene function. The symbiotic gene DNF2 encodes a putative phosphatidylinositol phospholipase C-like protein. Nodules formed by the mutant contain a zone of infected cells reduced to a few cell layers. In this zone, bacteria do not differentiate properly into bacteroids. Furthermore, mutant nodules senesce rapidly and exhibit defense-like reactions. This atypical phenotype amongst Fix(-) mutants unravels dnf2 as a new actor of bacteroid persistence inside symbiotic plant cells.
File Format	HTM / HTML
ISSN	0028646X
Issue Number	4
Volume Number	197
e-ISSN	14698137
Journal	New Phytologist
Language	English
Publisher	Wiley
Publisher Date	2013-03-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Botany Medicago Truncatula Microbiology Plant Proteins Physiology Sinorhizobium Symbiosis Genetics Gene Knockout Techniques Metabolism Nitrogen Fixation Phenotype Journal Article Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science

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