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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Copper, Christine L. Newman, Carl I. D. Collins, Greg E. |
| Description | Country affiliation: United States Author Affiliation: Copper CL ( United States Naval Academy, Chemistry Department, Annapolis, MD, USA.) |
| Abstract | Implementation of an uncomplicated SPE process for the rapid extraction and preconcentration of the alkaloids, colchicine, strychnine, aconitine, and nicotine, from water, apple juice, and nonfat milk samples is presented. When coupled to analysis via micellar EKC (MEKC), the total analysis time per sample was less than 15 min for the water and juice samples and less than 20 min for the milk. The SPE process allowed for anywhere from a three to a fourteen-fold improvement in the LOD for each alkaloid when compared to detecting the alkaloids in a nontreated water sample matrix. Following SPE, the LODs for colchicine, strychnine, and nicotine were sufficient to meet levels from 150 to 5000 times more dilute than the LD(50) for a 50 kg individual drinking 12 oz of a contaminated beverage. Aconitine, on the other hand, was detected at approximately the LD(50) level. The percent recoveries for the SPE ranged from 37% to as high as 99%. Nicotine attained the highest recovery efficiencies, followed by colchicine, and finally, aconitine and strychnine, which were nearly identical. The greatest recovery efficiencies were achieved from apple juice and water, whereas nonfat milk yielded the lowest. |
| File Format | HTM / HTML |
| ISSN | 16159306 |
| Issue Number | 21 |
| Volume Number | 31 |
| e-ISSN | 16159314 |
| Journal | Journal of Separation Science |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2008-12-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Alkaloids Analysis Beverages Chromatography, Micellar Electrokinetic Capillary Methods Spectrophotometry, Ultraviolet Aconitine Isolation & Purification Animals Colchicine Food Contamination Humans Molecular Structure Nicotine Sensitivity And Specificity Strychnine Evaluation Studies Journal Article Research Support, U.s. Gov't, Non-p.h.s. Research Support, U.s. Gov't, P.h.s. |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Filtration and Separation |
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