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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Otieno, Anthony C. Quainoo, Emmanuel W. Mwongela, Simon M. |
| Description | Country affiliation: United States Author Affiliation: Otieno AC ( Department of Chemistry, Kent State University, Kent, OH 44242, USA.) |
| Abstract | Phosphatidylinositol (PI) and its phosphorylated derivatives known as phosphoinositides (PIPs), are essential regulators of cell signaling and membrane trafficking, cytoskeletal dynamics, and nuclear functions. Disruption of PI metabolism is associated with disorders such as immune dysfunction, cardiovascular disease, and cancer; therefore, there is currently great interest in studying PIPs and their metabolic enzymes. Here, we describe a method for the separation of fluorescent PI and its seven fluorescent phosphorylated derivatives by CE-LIF. The CE method utilizes a Tris buffer and sodium deoxycholate in the presence of 30% 1-propanol and 5% of a dynamic coating reagent, EOTrol low reverse (EOTrol LR). It is simple, fast, highly sensitive, and it offers LODs in the order of 1.5 amol. The effect of cations such as lithium, sodium, potassium, cesium, barium, manganese, zinc, magnesium, calcium, spermine, and gentamicin were evaluated. Calcium and magnesium provided the best selectivity and resolution for the separation of the analytes while magnesium offered the best data reproducibility. The developed CE method would be useful in the studies of enzymatic activity in the PI and PIPs metabolic pathways using CE-based in vitro and CE cell-based assays, and/or for drug screening. |
| File Format | HTM / HTML |
| ISSN | 16159306 |
| Issue Number | 22 |
| Volume Number | 31 |
| e-ISSN | 16159314 |
| Journal | Journal of Separation Science |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2008-12-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Electrophoresis, Capillary Methods Fluorescent Dyes Analysis Phosphatidylinositols Calcium Chemistry Cations, Divalent Hydrogen-ion Concentration Magnesium Isolation & Purification Phosphorylation Reproducibility Of Results Temperature Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Filtration and Separation |
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