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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Stanley, Bob Mehr, Kevin A. Kellock, Trent Van Hamme, Jonathan D. Donkor, Kingsley K. |
| Description | Country affiliation: Canada Author Affiliation: Stanley B ( Department of Chemistry, Thompson Rivers University, Kamloops, BC., Canada.) |
| Abstract | A sensitive micellar electrokinetic chromatography (MEKC) method was developed for the separation and determination of four closely related lantibiotics: gallidermin, cinnamycin, duramycin and nisin. Factors affecting the separation of the lantibiotics such as pH, phosphate buffer concentration, SDS concentration and wavelength for UV detection were investigated. By optimizing these experimental conditions, successful separation was achieved between class 1A lantibiotics (nisin and gallidermin) and class 1B lantibiotics (duramycin and cinnamycin). The four lantibiotics were separated within 12 min in 50 mM phosphate buffer at pH 3.95 +/- 0.1 containing 80 mM SDS with UV detection of 214 nm. The LOD (S/N = 3) were 61 ng/mL for gallidermin, 57 ng/mL for cinnamycin, 55 ng/mL for duramycin and 58 ng/mL for nisin. The method was successfully applied to real samples such as fermentation broth, bovine colostrum and predrop beer. This method yielded satisfactory results, with quantitative recoveries of spiked lantibiotics in the three samples ranging from 86.1 to 99.6%. |
| File Format | HTM / HTML |
| ISSN | 16159306 |
| Issue Number | 17 |
| Volume Number | 32 |
| e-ISSN | 16159314 |
| Journal | Journal of Separation Science |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2009-09-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Anti-bacterial Agents Bacteriocins Amino Acid Sequence Animals Chemistry Isolation & Purification Beer Cattle Chromatography, Micellar Electrokinetic Capillary Instrumentation Methods Colostrum Molecular Sequence Data Nisin Peptides Peptides, Cyclic Reproducibility Of Results Sensitivity And Specificity Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Filtration and Separation |
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