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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Shi, Xueyan Liang, Pei Song, Dunlun Yang, Wenling Gao, Xiwu |
| Description | Author Affiliation: Shi X ( Department of Entomology, China Agricultural University, Beijing, PR China. shixueyan@cau.edu.cn) |
| Abstract | A novel method was developed for quantifying the levels of γ-aminobutyric acid (GABA) in the heads of houseflies (Musca domestica) and diamondback moths (Plutella xylostella (L.)), using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). The GABA in sample was derivatized with 4-chloro-7-nitro-2,1,3-benzoxadiazole (NBD-Cl) prior to CE-LIF analysis. In total, 32 mmol/L borate buffer, at pH 9.2 and containing 5.3 mmol/L ß-cyclodextrin (ß-CD) and 10.4 mmol/L sodium dodecyl sulfate (SDS), was determined to be the optimum CE background electrolyte (BGE) for GABA analysis. The detection limit of GABA was 0.016 µmol/L. The relative standard deviations (RSDs) of the migration time and peak area of GABA were 1.78 and 4.93%, respectively. The average recoveries of 0.97, 3.88, and 5.83 µmol/L of GABA, each added to the head sample of housefly, ranged from 88.9 to 110.5%. This method is simple and applicable to GABA assays of the heads of insects. With this newly developed CE-LIF method, the amounts of GABA in the heads of houseflies (M. domestica) and diamondback moths (P. xylostella (L.)) were measured. The results are relevant to the understandings of some insecticides and insecticide-resistance mechanisms in pests. |
| File Format | HTM / HTML |
| ISSN | 16159306 |
| Issue Number | 4 |
| Volume Number | 35 |
| e-ISSN | 16159314 |
| Journal | Journal of Separation Science |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2012-02-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Head Houseflies Anatomy & Histology Luminescent Measurements Methods Moths Gamma-aminobutyric Acid Analysis Animals Electrophoresis, Capillary Fluorescence Lasers Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Filtration and Separation |
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