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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Shaowen Sun, Xueping Wang, Wanhui Cai, Lingshuang |
| Description | Author Affiliation: Zhang S ( Department of Environmental Engineering and Chemistry, Luoyang Institute of Science and Technology, Luoyang, P R China. zhsw155@163.com) |
| Abstract | 8-Hydroxy-2'-deoxyguanosine (8-OHdG) is a sensitive biomarker for DNA oxidative damage. However, its determination in human urine is confounded by trace level and complex matrix. In this study, a new configuration of on-line solid phase microextraction coupled to high performance liquid chromatography-ultraviolet detection was established with molecularly imprinted monolithic column as extraction sorbent. The tailor made monolith exhibited high extraction efficiency with the enrichment factor 101.84 for 8-OHdG owing to its special porous structure and inherent selectivity. Under optimal condition, appreciable sensitivity had been achieved for this incorporation with limit of detection 2.04 nmol/L (S/N = 3) and limit of quantification 7.12 nmol/L (S/N = 10), respectively. Precise determination with wide range linearity (0.007-5.00 µmol/L) afforded a practical alternative in urinary 8-OHdG analysis and 107 different subjects had been successfully analyzed. This newly developed method embodied useful prospect for the investigation of DNA oxidative damage with less expense, convenient maintenance and ease of operation. |
| File Format | HTM / HTML |
| ISSN | 16159306 |
| Issue Number | 4 |
| Volume Number | 36 |
| e-ISSN | 16159314 |
| Journal | Journal of Separation Science |
| Language | English |
| Publisher | Wiley-VCH |
| Publisher Date | 2013-02-01 |
| Publisher Place | Germany |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Chromatography, High Pressure Liquid Methods Deoxyguanosine Urine Polymers Chemistry Solid Phase Microextraction Adsorption Instrumentation Isolation & Purification Humans Molecular Imprinting Chemical Synthesis Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Analytical Chemistry Filtration and Separation |
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