NDLI: Histidine-modified organic-silica hybrid monolithic column for mixed-mode per aqueous and ion-exchange capillary electrochromatography.

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Histidine-modified organic-silica hybrid monolithic column for mixed-mode per aqueous and ion-exchange capillary electrochromatography.

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Histidine-modified organic-silica hybrid monolithic column for mixed-mode per aqueous and ion-exchange capillary electrochromatography.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Tang, Sheng Liu, Shujuan Liang, Xiaojing Tang, Xiaofen Wu, Xingcai Guo, Yong Liu, Xia Jiang, Shengxiang
Description	Country affiliation: China Author Affiliation: Tang S ( Key Laboratory of Chemistry of Northwestern Plant Resources, CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.); Liu S ( University of Chinese Academy of Sciences, Chinese Academy of Sciences, Beijing, China.); Liang X ( Key Laboratory of Chemistry of Northwestern Plant Resources, CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.); Tang X ( Key Laboratory of Chemistry of Northwestern Plant Resources, CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.); Wu X ( Key Laboratory of Oil & Gas Production, China National Petroleum Corporation (CNPC) and Research Institute of Petroleum Exploration and Development (RIPED), Beijing, China.); Guo Y ( Key Laboratory of Oil & Gas Production, China National Petroleum Corporation (CNPC) and Research Institute of Petroleum Exploration and Development (RIPED), Beijing, China.); Liu X ( Key Laboratory of Chemistry of Northwestern Plant Resources, CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.); Jiang S ( Key Laboratory of Chemistry of Northwestern Plant Resources, CAS and Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, China.)
Abstract	A novel organic-silica hybrid monolith was prepared through the binding of histidine onto the surface of monolithic matrix for mixed-mode per aqueous and ion-exchange capillary electrochromatography. The imidazolium and amino groups on the surface of the monolithic stationary phase were used to generate an anodic electro-osmotic flow as well as to provide electrostatic interaction sites for the charged compounds at low pH. Typical per aqueous chromatographic behavior was observed in water-rich mobile phases. Various polar and hydrophilic analytes were selected to evaluate the characteristics and chromatographic performance of the obtained monolith. Under per aqueous conditions, the mixed-mode mechanism of hydrophobic and ion-exchange interactions was observed and the resultant monolithic column proved to be very versatile for the efficient separations of these polar and hydrophilic compounds (including amides, nucleosides and nucleotide bases, benzoic acid derivatives, and amino acids) in highly aqueous mobile phases. The successful applications suggested that the histidine-modified organic-silica hybrid monolithic column could offer a wide range of retention behaviors and flexible selectivities toward polar and hydrophilic compounds.
File Format	HTM / HTML
ISSN	16159306
Issue Number	12
Journal	Journal of Separation Science
Volume Number	38
e-ISSN	16159314
Language	English
Publisher	Wiley-VCH
Publisher Date	2015-06-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Content Type	Text
Resource Type	Article
Subject	Analytical Chemistry Filtration and Separation

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