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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Chu, E. S. M. Sze, S. C. W. Cheung, H. P. Wong, K. L. Liu, Q. Ng, T. B. Tong, Y. |
| Description | Country affiliation: China Author Affiliation: Chu ES ( School of Chinese Medicine, LKS Faculty of Medicine, The University of Hong Kong, 10 Sassoon Road, Pokfulam, Hong Kong, China.) |
| Abstract | AIM OF STUDY: This study aimed to elucidate and compare the anti-metastatic mechanism of Tian-Xian liquid (TXL) and its bioactive components namely butanol (BU), ethyl-acetate (EA) and aqueous (WA) fractions on human colorectal cancer in vitro (HT-29 cancer cells) and in vivo (nude mouse xenografts). MATERIALS AND METHODS: The anti-proliferative effects of TXL and its bioactive components in HT-29 cells were determined by MTT assay. Their modulations on the potential angiogenic and metastatic marker expressions on HT-29 cells and xenografts were investigated by real-time PCR and Western blot at transcriptional and translational levels, respectively. For the in vitro study, migration abilities of HT-29 cells were determined using wound healing assay. For the in vivo study, daily measurements of the tumor size and volume of the xenografts were also performed. RESULTS: TXL, BU, EA and WA effectively inhibited the proliferation of HT-29 cells in a dose- and time-dependent manner. The IC(50) value of TXL on HT-29 cells was obtained after incubation with 1% (v/v) TXL for 4h; whereas IC(50) values were obtained for the following bioactive components: BU at 1.25% (v/v); EA at 5% (v/v); and WA at 0.3125% (v/v). It was found that 1% (v/v) TXL significantly down-regulated MMP2 and MMP7 expression at both transcriptional and translational levels and it reduced MMP9 and VEGF protein expression in vitro. TXL decreased the metastatic ability of HT-29 cells as demonstrated by wound healing assay. TXL and its bioactive fractions caused no significant changes in the body weight indicating lack of toxicity to the xenografts. CONCLUSIONS: In summary, TXL multi-targeted to down-regulate the metastatic markers in both in vitro and in vivo models. However, the effects of its bioactive fractions were not obvious. This study profoundly elucidated the anti-proliferative mechanism of TXL, which is vital for the development of future anti-cancer regime in Chinese medicinal formulations. |
| File Format | HTM / HTML |
| ISSN | 03788741 |
| Issue Number | 1 |
| Volume Number | 137 |
| Journal | Journal of Ethnopharmacology |
| e-ISSN | 18727573 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2011-09-01 |
| Publisher Place | Ireland |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Ethnopharmacology Antineoplastic Agents, Phytogenic Pharmacology Colorectal Neoplasms Drug Therapy Drugs, Chinese Herbal Acetates Chemistry Animals Blotting, Western Butanols Cell Movement Drug Effects Cell Proliferation Chemical Fractionation Genetics Metabolism Secondary Dose-response Relationship, Drug Female Gene Expression Regulation, Neoplastic Ht29 Cells Humans Inhibitory Concentration 50 Matrix Metalloproteinase 2 Matrix Metalloproteinase 7 Matrix Metalloproteinase 9 Mice Mice, Nude Neoplasm Invasiveness Real-time Polymerase Chain Reaction Reverse Transcriptase Polymerase Chain Reaction Solvents Time Factors Tumor Burden Vascular Endothelial Growth Factor A Water Xenograft Model Antitumor Assays Comparative Study Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmacology |
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