NDLI: Rho-kinase mediated cytoskeletal stiffness in skinned smooth muscle.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lan, Bo Wang, Lu Zhang, Jenny Pascoe, Chris D. Norris, Brandon A. Liu, Jeffrey C-Y Solomon, Dennis Paré, Peter D. Deng, Linhong Seow, Chun Y.
Description	Author Affiliation: Lan B ( Bioengineering College, Chongqing University, Chongqing, China)
Abstract	The structurally dynamic cytoskeleton is important in many cell functions. Large gaps still exist in our knowledge regarding what regulates cytoskeletal dynamics and what underlies the structural plasticity. Because Rho-kinase is an upstream regulator of signaling events leading to phosphorylation of many cytoskeletal proteins in many cell types, we have chosen this kinase as the focus of the present study. In detergent skinned tracheal smooth muscle preparations, we quantified the proteins eluted from the muscle cells over time and monitored the muscle's ability to respond to acetylcholine (ACh) stimulation to produce force and stiffness. In a partially skinned preparation not able to generate active force but could still stiffen upon ACh stimulation, we found that the ACh-induced stiffness was independent of calcium and myosin light chain phosphorylation. This indicates that the myosin light chain-dependent actively cycling crossbridges are not likely the source of the stiffness. The results also indicate that Rho-kinase is central to the ACh-induced stiffness, because inhibition of the kinase by H1152 (1 µM) abolished the stiffening. Furthermore, the rate of relaxation of calcium-induced stiffness in the skinned preparation was faster than that of ACh-induced stiffness, with or without calcium, suggesting that different signaling pathways lead to different means of maintenance of stiffness in the skinned preparation.
File Format	HTM / HTML
ISSN	87507587
e-ISSN	15221601
DOI	10.1152/japplphysiol.00654.2013
Journal	Journal of Applied Physiology
Issue Number	10
Volume Number	115
Language	English
Publisher	American Physiological Society
Publisher Date	2013-11-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physiology Cytoskeleton Enzymology Muscle Contraction Myocytes, Smooth Muscle Trachea Rho-associated Kinases Metabolism 1-(5-isoquinolinesulfonyl)-2-methylpiperazine Analogs & Derivatives Pharmacology Acetylcholine Animals Calcium Cell Shape Elasticity Drug Effects Myosin Light Chains Phosphorylation Protein Kinase Inhibitors Signal Transduction Time Factors Cytology Antagonists & Inhibitors Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Physiology Physiology (medical) Sports Science

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