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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Riva, A. Puxeddu, P. Del Fiacco, M. Testa-Riva, F. |
| Description | Country affiliation: Italy Author Affiliation: Riva A ( Istituto di Anatomia Umana Normale, Università di Cagliari, 09100 Cagliari, Italy.) |
| Abstract | The localization of endogenous peroxidase was studied in human parotid and submandibular glands using the medium of Strum & Karnovsky either at pH 7 or at pH 8.3, after a short fixation of the tissues with a low concentration of glutaraldehyde. At pH 7 reaction product was found in seromucous cells of both glands in the cisternae of the rough endoplasmic reticulum, in the elements of the Golgi apparatus, and in secretory products. However, while in the parotid gland all secretory granules showed peroxidase activity, in the submandibular gland some of the granules were left unstained. At pH 8.3 the pattern of reactivity was practically unchanged in the submandibular gland, whereas the amount of precipitate noticeable in the secretory granules of the parotid gland was greatly reduced. The method of Novikoff et al. for catalase revealed the presence of a number of small bodies having in the characteristics of peroxisomes. Acid phosphatase was not demonstrable in the secretory granules of either parotid or submandibular glands. The lead precipitate indicating enzyme activity was observed only in lysosomes and, occasionally, in the elements of the Golgi apparatus of some sermucous cells. |
| File Format | HTM / HTML |
| ISSN | 14697580 |
| e-ISSN | 14697580 |
| Journal | Journal of Anatomy |
| Issue Number | Pt 1 |
| Volume Number | 127 |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 1978-09-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Discipline Anatomy Parotid Gland Enzymology Peroxidases Submandibular Gland Adolescent Endoplasmic Reticulum Golgi Apparatus Histocytochemistry Microscopy, Electron Ultrastructure |
| Content Type | Text |
| Resource Type | Article |
| Subject | Anatomy Cell Biology Developmental Biology Histology Molecular Biology Ecology, Evolution, Behavior and Systematics |
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