NDLI: Immunocytochemical characterisation of olfactory ensheathing cells of zebrafish.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lazzari, Maurizio Bettini, Simone Franceschini, Valeria
Description	Country affiliation: Italy Author Affiliation: Lazzari M ( Department of Biological, Geological and Environmental Sciences, University of Bologna, Bologna, Italy.)
Abstract	Continuous lifelong neurogenesis is typical of the vertebrate olfactory system. The regenerative ability of olfactory receptor neurons is dependent on the glial cell type specific to the olfactory pathway, designated 'olfactory ensheathing cells'. Several studies to date have focused on mammalian olfactory ensheathing cells, owing to their potential roles in cell-based therapy for spinal cord injury repair. However, limited information is available regarding this glial cell type in non-mammalian vertebrates, particularly anamniotes. In the current immunocytochemical study, we analysed the features of olfactory ensheathing cells in the zebrafish, Danio rerio. Fish provide a good model for studying glial cells associated with the olfactory pathway of non-mammalian vertebrates. In particular, zebrafish has numerous valuable features that enable its use as a prime model organism for genetic, neurobiological and developmental studies, as well as toxicology and genomics research. Paraffin sections from decalcified heads of zebrafish were processed immunocytochemically to detect proteins used in the research on mammalian olfactory ensheathing cells, including glial fibrillary acid protein (GFAP), S100, neural cell adhesion molecule (NCAM), polysialylated NCAM (PSA-NCAM), vimentin (VIM), p75NTR and galactin (Gal)-1. Notably, GFAP, S100, NCAM and Gal-1 were clearly observed, whereas no vimentin staining was detected. Weak immunostaining for PSA-NCAM and p75NTR was evident. Moreover the degree of marker expression was not uniform in various tracts of the zebrafish olfactory pathway. The immunostaining patterns of the zebrafish olfactory system are distinct from those of other fish to some extent, suggesting interspecific differences. We also showed that the olfactory pathway of zebrafish expresses markers of mammalian olfactory ensheathing cells. The olfactory systems of vertebrates have similarities but there are also marked variations between them. The issue of whether regional and interspecific differences in immunostaining patterns of olfactory pathway markers have functional significance requires further investigation.
File Format	HTM / HTML
ISSN	14697580
e-ISSN	14697580
DOI	10.1111/joa.12129
Journal	Journal of Anatomy
Issue Number	2
Volume Number	224
Language	English
Publisher	Wiley
Publisher Date	2014-02-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Anatomy Olfactory Bulb Cytology Olfactory Pathways Animals Biological Markers Immunohistochemistry Chemistry Olfactory Receptor Neurons S100 Proteins Zebrafish Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Anatomy Cell Biology Developmental Biology Histology Molecular Biology Ecology, Evolution, Behavior and Systematics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Immunocytochemical characterisation of olfactory ensheathing cells of zebrafish

Differential bulbar and extrabulbar projections of diverse olfactory receptor neuron populations in the adult zebrafish (Danio rerio).

Ruffed cells in the olfactory bulb of freshwater teleosts. I. Golgi impregnation.

The vomeronasal organ of the cat.

Calretinin in the peripheral nervous system of the adult zebrafish.

Comparative morphology of the accessory olfactory bulb in bats.

Ultrastructural characterisation of the olfactory mucosa of the armadillo Dasypus hybridus (Dasypodidae, Xenarthra).

Cell proliferation in the olfactory bulb of adult freshwater teleosts.

Localised degeneration occurs in aged mouse olfactory epithelium.

Immunocytochemical characterisation of olfactory ensheathing cells of zebrafish.

Similar Documents

Immunocytochemical characterisation of olfactory ensheathing cells of zebrafish

Differential bulbar and extrabulbar projections of diverse olfactory receptor neuron populations in the adult zebrafish (Danio rerio).

Ruffed cells in the olfactory bulb of freshwater teleosts. I. Golgi impregnation.

The vomeronasal organ of the cat.

Calretinin in the peripheral nervous system of the adult zebrafish.

Comparative morphology of the accessory olfactory bulb in bats.

Ultrastructural characterisation of the olfactory mucosa of the armadillo Dasypus hybridus (Dasypodidae, Xenarthra).

Cell proliferation in the olfactory bulb of adult freshwater teleosts.

Localised degeneration occurs in aged mouse olfactory epithelium.

Immunocytochemical characterisation of olfactory ensheathing cells of zebrafish.