| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Chen, Yun-Yun K. Khoury, Paneez Ware, JeanAnne M. Holland-Thomas, Nicole C. Stoddard, Jennifer L. Gurprasad, Shakuntala Waldner, Amy J. Klion, Amy D. |
| Description |
Country affiliation: Moldova Author Affiliation: Chen YY ( Eosinophil Pathology Unit, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Frederick, Md.); Khoury P ( Eosinophil Pathology Unit, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Frederick, Md.); Ware JM ( Eosinophil Pathology Unit, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Frederick, Md.); Holland-Thomas NC ( Laboratory of Parasitic Diseases, Clinical Research Directorate/CMRP, SAIC-Frederick, Inc, Frederick National Laboratory for Cancer Research, Frederick, Md.); Stoddard JL ( Department of Laboratory Medicine, Warren Magnusson Clinical Center, National Institutes of Health, Bethesda, Md.); Gurprasad S ( Department of Laboratory Medicine, Warren Magnusson Clinical Center, National Institutes of Health, Bethesda, Md.); Waldner AJ ( Eosinophil Pathology Unit, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Frederick, Md.); Klion AD ( Eosinophil Pathology Unit, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, Frederick, Md. Electronic address: aklion@nih.gov.) |
| Abstract |
BACKGROUND: Although most patients with hypereosinophilic syndromes (HES) present with clinical signs and symptoms attributable to eosinophilic tissue infiltration, some untreated patients remain asymptomatic or have signs and symptoms, such as allergic rhinitis, for which the relationship to peripheral eosinophilia is unclear (hypereosinophilia of unknown significance [HEUS]). OBJECTIVE: To identify and characterize subjects with HEUS of 5 years duration or more as compared to untreated patients with symptomatic HES and healthy normal volunteers. METHODS: All subjects with eosinophilia underwent yearly evaluation, including a standardized clinical evaluation, whole blood flow cytometry to assess lymphocyte subsets and eosinophil activation, and serum collection. Peripheral blood mononuclear cells were cultured overnight with and without phorbol 12-myristate 13-acetate/ionomycin. Cytokines and chemokines were measured in serum and cell supernatants, and mRNA expression was assessed by using quantitative real-time PCR. RESULTS: Eight of the 210 subjects referred for the evaluation of eosinophilia (absolute eosinophil count [AEC] > 1500/µL) met the criteria for HEUS of 5 years duration or more (range, 7-29 years). Peak eosinophil count and surface expression of eosinophil activation markers were similar in subjects with HEUS and in untreated subjects with platelet-derived growth factor alpha-negative HES (n = 28). Aberrant or clonal T-cell populations were identified in 50% of the subjects with HEUS as compared to 29% of the subjects with HES (P = .12). Increased levels of IL-5, GM-CSF, IL-9, and IL-17A were also comparable in subjects with HEUS and HES. Serum levels of IgE and IL-13 were significantly increased only in subjects with HES. CONCLUSIONS: A small number of patients with persistent peripheral eosinophilia (AEC > 1500/µL) appear to have clinically benign disease. |
| File Format |
HTM / HTML |
| ISSN |
00916749 |
| e-ISSN |
10976825 |
| DOI |
10.1016/j.jaci.2013.06.037 |
| Journal |
Journal of Allergy and Clinical Immunology |
| Issue Number |
4 |
| Volume Number |
133 |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2014-04-01 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Discipline Immunology Eosinophilia Diagnosis Adolescent Chemokine Ccl17 Blood Genetics Cytokines Eosinophils Immunology Metabolism Hypereosinophilic Syndrome Leukocyte Count Phenotype T-lymphocytes Research Support, N.i.h., Extramural Research Support, N.i.h., Intramural |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Immunology and Allergy Immunology |
