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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Ke, Chunlin Qiao, Deliang Luo, Jianguang Li, Zuomei Sun, Yi Ye, Hong Zeng, Xiaoxiong |
| Description | Author Affiliation: Ke C ( College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, PR China.) |
| Abstract | In the present study, crude capsule polysaccharides from Streptococcus equi subsp. zooepidemicus C55129 (CP) were purified by DEAE cellulose-52 column and Sephadex G-100 column chromatography to afford purified fractions of CP-1, CP-2 and CP-3. The relative molecular weights of CP-1, CP-2 and CP-3 were 29.0, 891.0 and 1338.0 kDa, respectively. CP-1 was composed of mannose, glucose, arabinose and galactose in a percentage ratio of 66.15:28.97:2.43:2.45. CP-2 was composed of mannose, glucose, galactose and glucuronic acid in a percentage ratio of 40.94:27.71:5.96:25.39. In CP-1, there were pyranose rings, -configuration glycosidic bond residues, one backbone chain (1â6 glycosidic bonds) and two branch chains (1â3 and 1â4 glycosidic bonds) in one repeating unit. In vitro immunostimulatory assay, it was found that CP could promote the splenocyte proliferation and increase the activity of acid phosphatase in peritoneal macrophages. The immunostimulatory activity of CP-1 might be related to its monosaccharide composition, molecular weight and -configuration glycosidic bond. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Volume Number | 57 |
| e-ISSN | 18790003 |
| Journal | International Journal of Biological Macromolecules |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-06-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Adjuvants, Immunologic Cell Proliferation Drug Effects Macrophages, Peritoneal Metabolism Polysaccharides, Bacterial Spleen Streptococcus Equi Chemistry Acid Phosphatase Isolation & Purification Pharmacology Animals Cells, Cultured Cytology Mice Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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