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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Gomba, Gordon Karikoga Synytsya, Andriy Svecová, Petra Coimbra, Manuel A. Copíková, Jana |
| Description | Author Affiliation: Gomba GK ( Department of Carbohydrates and Cereals, University of Chemistry and Technology, Prague, Technická 5, 166 28, Prague 6, Czech Republic. Electronic address: gkgomba@vscht.cz.); Synytsya A ( Department of Carbohydrates and Cereals, University of Chemistry and Technology, Prague, Technická 5, 166 28, Prague 6, Czech Republic. Electronic address: sinicaa@vscht.cz.); Svecová P ( Department of Carbohydrates and Cereals, University of Chemistry and Technology, Prague, Technická 5, 166 28, Prague 6, Czech Republic. Electronic address: PetraBlafkova@seznam.cz.); Coimbra MA ( Departamento de Química, Universidade de Aveiro, Campus de Santiago, 3810-193 Aveiro, Portugal. Electronic address: copikovj@vscht.cz.); Copíková J ( Department of Carbohydrates and Cereals, University of Chemistry and Technology, Prague, Technická 5, 166 28, Prague 6, Czech Republic. Electronic address: mac@ua.pt.) |
| Abstract | A set of fungal polysaccharide samples was characterised by elemental analysis and FTIR spectroscopy and compared with reference chitins, chitosans and ß-D-glucans. The nitrogen to carbon (N/C) values and FTIR spectra were used to compare the samples based on their composition. It was found that the N/C ratio correlates well with deacetylation degree (DD) of chitosans and chitin/glucan ratio R(chit) of fungal chitin ß-D-glucan complexes with the exception of some samples having significant nitrogen and/or carbon admixtures. FTIR spectroscopy was indicative for the N-acetylation of chitins (chitosans) as well as for the chitin (chitosan) contribution to fungal polysaccharide preparations. Multivariate analyses of the FTIR data (HCA, PCA) discriminated samples and reference materials into several clusters depending on their similarity. Chitosan lactates, chitosan ß-D-glucans and chitin ß-D-glucans of high and low amounts of chitin were successfully discriminated from the reference polysaccharides and from each other. The proposed procedures based on the N/C ratio and multivariate analyses of FTIR spectra may be used in screening fungal polysaccharide preparations. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Journal | International Journal of Biological Macromolecules |
| Volume Number | 80 |
| e-ISSN | 18790003 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-09-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biochemistry Carbon Chemistry Fungal Polysaccharides Nitrogen Aspergillus Niger Species Specificity Spectrophotometry, Infrared Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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