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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Jamshidian, Hajar Shojaosadati, Seyed Abbas Vilaplana, Francisco Mousavi, Seyed Mohammad Soudi, Mohammad Reza |
| Description | Country affiliation: Iran Author Affiliation: Jamshidian H ( Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, 14115-143 Tehran, Iran.); Shojaosadati SA ( Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, 14115-143 Tehran, Iran. Electronic address: shoja_sa@modares.ac.ir.); Vilaplana F ( Division of Glycoscience, School of Biotechnology, KTH Royal Institute of Technology, AlbaNova University Centre, SE-10691 Stockholm, Sweden.); Mousavi SM ( Biotechnology Group, Chemical Engineering Department, Tarbiat Modares University, 14115-143 Tehran, Iran.); Soudi MR ( Microbiology Department, Faculty of Biological Sciences, Alzahra University, 19938-91176 Tehran, Iran.) |
| Abstract | This study demonstrates the efficient utilization of low-cost agricultural substrates, particularly date syrup, by Schizophyllum commune ATCC 38548 for schizophyllan production. Initially, one factor-at-a-time method was used to find the best carbon and nitrogen sources for schizophyllan production. Subsequently, response surface methodology was employed to optimize the level of culture medium components to maximize substrate conversion yield and schizophyllan production in submerged culture. Maximum product yield (0.12g schizophyllan/g date syrup) and schizophyllan production (8.5g/l) were obtained at concentrations of date syrup and corn steep liquor, inoculum size and agitation rate at 7.02%w/v, 0.10%w/v, 7.68%v/v and 181rpm, respectively. Sugar composition analysis, FTIR, NMR and molar mass determination revealed the purity and molecular properties of recovered schizophyllan produced from date syrup as glycosidic linkage analysis showed three main schizophyllan characteristic peaks arising from the 3-linked, 3,6-linked and terminal glucose residues. Finally, process economic analysis suggested that use of date syrup and corn steep liquor as nutrients would result in approximately 6-fold reduction in cost of raw materials for schizophyllan production as compared to conventional carbon and nitrogen sources such as sucrose and malt extract. |
| File Format | HTM / HTML |
| ISSN | 01418130 |
| Journal | International Journal of Biological Macromolecules |
| Volume Number | 92 |
| e-ISSN | 18790003 |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2016-11-01 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Content Type | Text |
| Resource Type | Article |
| Subject | Structural Biology Molecular Biology Biochemistry |
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