NDLI: Multiepitope peptide-loaded virus-like particles as a vaccine against hepatitis B virus-related hepatocellular carcinoma.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ding, Fei-Xiang Wang, Fang Lu, Yi-Ming Li, Ka Wang, Kai-Hui He, Xiao-Wen Sun, Shu-Han
Description	Author Affiliation: Ding FX ( Department of Medical Genetics, The Second Military Medical University, Shanghai, People's Republic of China.)
Abstract	UNLABELLED: To develop a hepatitis B virus (HBV) therapeutic vaccine that can induce a broad but specific immune response and significant antitumor effects both in vivo and in vitro, we inserted HBV X protein (HBx)-derived epitopes HBx(52-60), HBx(92-100), and HBx(115-123); a novel subdominant cytolytic T lymphocyte (CTL) epitope HBx(140-148); and the universal T helper epitope pan human leukocyte antigen DR-binding epitope into HBV core protein to form multiepitope peptide-loaded virus-like particles (VLPs). CTL responses against epitope-loaded VLPs were elicited by priming with VLP-pulsed dendritic cells in both HLA-A*0201 transgenic (Tg) mice and peripheral blood lymphocytes from HLA-A2(+)/HBx(+) HBV-infected hepatocellular carcinoma (HCC) patients. The multiepitope peptide-loaded VLPs demonstrated significantly higher immunogenicity in Tg mice than any single responsive epitope. Significant antitumor effects were demonstrated both with primary cultured autologous HCC cells in vitro and tumor-bearing Tg mice in vivo in an HLA-A2-restricted and epitope-specific fashion. CONCLUSION: The significant antitumor effects both in vivo and in vitro demonstrate the potential of multiepitope peptide-loaded VLPs as a vaccine against HCC.
File Format	HTM / HTML
ISSN	02709139
Issue Number	5
Volume Number	49
e-ISSN	15273350
Journal	Hepatology
Language	English
Publisher	Wiley
Publisher Date	2009-05-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Hepatology Carcinoma, Hepatocellular Immunology Epitopes, T-lymphocyte Hepatitis B Vaccines Biosynthesis Liver Neoplasms Trans-activators Viral Regulatory And Accessory Proteins Adoptive Transfer Animals Virology Cell Line, Tumor Cytotoxicity, Immunologic Dendritic Cells Hla-a Antigens Metabolism Hla-a2 Antigen Humans Mice Mice, Transgenic Protein Stability Vaccination Vaccines, Subunit Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Hepatology

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