NDLI: Alpha2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III.

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Alpha2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III.

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Null mutation of mCOUP-TFI results in defects in morphogenesis of the glossopharyngeal ganglion, axonal projection, and arborization.

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Mutations in Drosophila DP and E2F distinguish G1-S progression from an associated transcriptional program.

The response to extracytoplasmic stress in Escherichia coli is controlled by partially overlapping pathways.

Alpha2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Szeto, L. Fafalios, M. K. Zhong, H. Vershon, A. K. Broach, J. R.
Description	Country affiliation: United States Author Affiliation: Szeto L ( Department of Molecular Biology, Princeton University, New Jersey 08544-1014, USA.)
Abstract	Homothallic strains of Saccharomyces cerevisiae can change mating type as often as every generation by replacing the allele at the MAT locus with a copy of mating type information present at one of two storage loci, HML and HMR, located on either end of chromosome III. Selection of the appropriate donor locus is dictated by a mating type-specific repressor protein, alpha2p: Cells containing alpha2p select HMR, whereas those lacking alpha2p select HML. As a repressor protein, alpha2p binds to DNA cooperatively with the transcriptional activator Mcm1p. Here we show that two alpha2p/Mcm1p-binding sites, DPS1 and DPS2, control donor selection. DPS1 and DPS2 are located approximately 30 kb from the left arm of chromosome III, well removed from HML, HMR, and MAT. Precise deletion of only DPS1 and DPS2 results in random selection of donor loci and in a cells without affecting selection in alpha cells. Reciprocally, deletion of only the alpha2p binding segments in each of these two sites results in selection of the wrong donor loci in alpha cells without affecting preference in a cells. These results suggest that Mcm1p, bound to these two sites in the absence of alpha2p, activates HML as donor. Binding of alpha2p blocks the ability of Mcm1p bound to DPS1 and DPS2 to activate HML, resulting in default selection of HMR as donor. DPS1 and DPS2 also regulate expression of several noncoding RNAs, although deletion of at least one of these RNA loci does not affect donor preference. This suggests that transcriptional activation, rather than transcription of a specific product, is the initiating event in activating the left arm of chromosome III for donor selection.
File Format	HTM / HTML
ISSN	08909369
e-ISSN	15495477
Journal	Genes & Development
Issue Number	15
Volume Number	11
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	1997-08-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Molecular Discipline Biology__semicolon__developmental Discipline Biology__semicolon__genetics__semicolon__cell Discipline Biology Chromosomes, Fungal Genetics Enhancer Elements, Genetic Homeodomain Proteins Metabolism Recombination, Genetic Repressor Proteins Saccharomyces Cerevisiae Binding Sites Dna, Fungal Dna-binding Proteins Gene Expression Regulation, Fungal Minichromosome Maintenance 1 Protein Rna, Fungal Rna, Messenger Physiology Saccharomyces Cerevisiae Proteins Sequence Deletion Transcription Factors Transcriptional Activation Research Support, U.s. Gov't, P.h.s.
Content Type	Text
Resource Type	Article
Subject	Genetics Developmental Biology

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Saccharomyces forkhead protein Fkh1 regulates donor preference during mating-type switching through the recombination enhancer

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Alpha2p controls donor preference during mating type interconversion in yeast by inactivating a recombinational enhancer of chromosome III.

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