| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Kapoor, Prabodh Bao, Yunhe Xiao, Jing Luo, Jie Shen, Jianfeng Persinger, Jim Peng, Guang Ranish, Jeff Bartholomew, Blaine Shen, Xuetong |
| Description |
Author Affiliation: Kapoor P ( Department of Epigenetics and Molecular Carcinogenesis, The University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, USA); Bao Y ( Department of Epigenetics and Molecular Carcinogenesis, The University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, USA); Xiao J ( Department of Epigenetics and Molecular Carcinogenesis, The University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, USA); Luo J ( Institute for Systems Biology, Seattle, Washington 98109, USA); Shen J ( Department of Clinical Cancer Prevention, The University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA.); Persinger J ( Department of Epigenetics and Molecular Carcinogenesis, The University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, USA); Peng G ( Department of Clinical Cancer Prevention, The University of Texas M.D. Anderson Cancer Center, Houston, Texas 77030, USA.); Ranish J ( Institute for Systems Biology, Seattle, Washington 98109, USA); Bartholomew B ( Department of Epigenetics and Molecular Carcinogenesis, The University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, USA); Shen X ( Department of Epigenetics and Molecular Carcinogenesis, The University of Texas M.D. Anderson Cancer Center, Smithville, Texas 78957, USA) |
| Abstract |
ATP-dependent chromatin remodeling complexes alter chromatin structure through interactions with chromatin substrates such as DNA, histones, and nucleosomes. However, whether chromatin remodeling complexes have the ability to regulate nonchromatin substrates remains unclear. Saccharomyces cerevisiae checkpoint kinase Mec1 (ATR in mammals) is an essential master regulator of genomic integrity. Here we found that the SWI/SNF chromatin remodeling complex is capable of regulating Mec1 kinase activity. In vivo, Mec1 activity is reduced by the deletion of Snf2, the core ATPase subunit of the SWI/SNF complex. SWI/SNF interacts with Mec1, and cross-linking studies revealed that the Snf2 ATPase is the main interaction partner for Mec1. In vitro, SWI/SNF can activate Mec1 kinase activity in the absence of chromatin or known activators such as Dpb11. The subunit requirement of SWI/SNF-mediated Mec1 regulation differs from that of SWI/SNF-mediated chromatin remodeling. Functionally, SWI/SNF-mediated Mec1 regulation specifically occurs in S phase of the cell cycle. Together, these findings identify a novel regulator of Mec1 kinase activity and suggest that ATP-dependent chromatin remodeling complexes can regulate nonchromatin substrates such as a checkpoint kinase. |
| File Format |
HTM / HTML |
| ISSN |
08909369 |
| e-ISSN |
15495477 |
| DOI |
10.1101/gad.257626.114 |
| Journal |
Genes & Development |
| Issue Number |
6 |
| Volume Number |
29 |
| Language |
English |
| Publisher |
Cold Spring Harbor Laboratory Press |
| Publisher Date |
2015-03-15 |
| Publisher Place |
United States |
| Access Restriction |
Open |
| Subject Keyword |
Discipline Molecular Discipline Biology__semicolon__developmental Discipline Biology__semicolon__genetics__semicolon__cell Discipline Biology Chromatin Metabolism Chromosomal Proteins, Non-histone Intracellular Signaling Peptides And Proteins Protein-serine-threonine Kinases Saccharomyces Cerevisiae Proteins Saccharomyces Cerevisiae Enzymology Genetics Transcription Factors Adenosine Triphosphatases Chromatin Assembly And Disassembly Dna Damage Physiology Enzyme Activation Enzyme Activators S Phase Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Genetics Developmental Biology |
