NDLI: Chaperoning 5S RNA assembly.

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Chaperoning 5S RNA assembly.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Madru, Clément Lebaron, Simon Blaud, Magali Delbos, Lila Pipoli, Juliana Pasmant, Eric Réty, Stéphane Leulliot, Nicolas
Description	Author Affiliation: Madru C ( Laboratoire de Cristallographie et RMN Biologiques, UMR, CNRS 8015, Université Paris Descartes, Faculté de Pharmacie, Sorbonne Paris Cité, 75006 Paris, France); Lebaron S ( Laboratoire de Cristallographie et RMN Biologiques, UMR, CNRS 8015, Université Paris Descartes, Faculté de Pharmacie, Sorbonne Paris Cité, 75006 Paris, France); Blaud M ( Laboratoire de Cristallographie et RMN Biologiques, UMR, CNRS 8015, Université Paris Descartes, Faculté de Pharmacie, Sorbonne Paris Cité, 75006 Paris, France); Delbos L ( Laboratoire de Cristallographie et RMN Biologiques, UMR, CNRS 8015, Université Paris Descartes, Faculté de Pharmacie, Sorbonne Paris Cité, 75006 Paris, France); Pipoli J ( Plateforme Génomique, Institut Cochin, UMR_S1016, INSERM, 75006 Paris, France); Pasmant E ( Service de Biochimie et Génétique Moléculaire, Hôpital Cochin, Assistance Publique-Hôpitaux de Paris, 75006 Paris, France.); Réty S ( Laboratoire de Cristallographie et RMN Biologiques, UMR, CNRS 8015, Université Paris Descartes, Faculté de Pharmacie, Sorbonne Paris Cité, 75006 Paris, France); Leulliot N ( Laboratoire de Cristallographie et RMN Biologiques, UMR, CNRS 8015, Université Paris Descartes, Faculté de Pharmacie, Sorbonne Paris Cité, 75006 Paris, France)
Abstract	In eukaryotes, three of the four ribosomal RNAs (rRNAs)the 5.8S, 18S, and 25S/28S rRNAsare processed from a single pre-rRNA transcript and assembled into ribosomes. The fourth rRNA, the 5S rRNA, is transcribed by RNA polymerase III and is assembled into the 5S ribonucleoprotein particle (RNP), containing ribosomal proteins Rpl5/uL18 and Rpl11/uL5, prior to its incorporation into preribosomes. In mammals, the 5S RNP is also a central regulator of the homeostasis of the tumor suppressor p53. The nucleolar localization of the 5S RNP and its assembly into preribosomes are performed by a specialized complex composed of Rpf2 and Rrs1 in yeast or Bxdc1 and hRrs1 in humans. Here we report the structural and functional characterization of the Rpf2-Rrs1 complex alone, in complex with the 5S RNA, and within pre-60S ribosomes. We show that the Rpf2-Rrs1 complex contains a specialized 5S RNA E-loop-binding module, contacts the Rpl5 protein, and also contacts the ribosome assembly factor Rsa4 and the 25S RNA. We propose that the Rpf2-Rrs1 complex establishes a network of interactions that guide the incorporation of the 5S RNP in preribosomes in the initial conformation prior to its rotation to form the central protuberance found in the mature large ribosomal subunit.
File Format	HTM / HTML
ISSN	08909369
e-ISSN	15495477
DOI	10.1101/gad.260349.115
Journal	Genes & Development
Issue Number	13
Volume Number	29
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2015-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Molecular Discipline Biology__semicolon__developmental Discipline Biology__semicolon__genetics__semicolon__cell Discipline Biology Models, Molecular Nuclear Proteins Chemistry Rna, Ribosomal, 5s Metabolism Rna-binding Proteins Saccharomyces Cerevisiae Proteins Binding Sites Cryoelectron Microscopy Protein Binding Protein Structure, Quaternary Saccharomyces Cerevisiae Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Genetics Developmental Biology

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