| Author |
Zentella, Rodolfo Hu, Jianhong Hsieh, Wen-Ping Matsumoto, Peter A. Dawdy, Andrew Barnhill, Benjamin Oldenhof, Harriëtte Hartweck, Lynn M. Maitra, Sushmit Thomas, Stephen G. Cockrell, Shelley Boyce, Michael Shabanowitz, Jeffrey Hunt, Donald F. Olszewski, Neil E. Sun, Tai-Ping |
| Description |
Author Affiliation: Zentella R ( Department of Biology, Duke University, Durham, North Carolina 27708, USA); Hu J ( Department of Biology, Duke University, Durham, North Carolina 27708, USA); Hsieh WP ( Department of Biology, Duke University, Durham, North Carolina 27708, USA); Matsumoto PA ( Department of Plant Biology, University of Minnesota, Saint Paul, Minnesota 55108, USA); Dawdy A ( Department of Chemistry, University of Virginia, Charlottesville, Virginia 22901, USA); Barnhill B ( Department of Chemistry, University of Virginia, Charlottesville, Virginia 22901, USA); Oldenhof H ( Department of Plant Biology, University of Minnesota, Saint Paul, Minnesota 55108, USA); Hartweck LM ( Department of Plant Biology, University of Minnesota, Saint Paul, Minnesota 55108, USA); Maitra S ( Department of Chemistry, University of Virginia, Charlottesville, Virginia 22901, USA); Thomas SG ( Department of Biology, Duke University, Durham, North Carolina 27708, USA); Cockrell S ( Department of Biology, Duke University, Durham, North Carolina 27708, USA); Boyce M ( Department of Biochemistry, Duke University School of Medicine, Durham, North Carolina 27710, USA); Shabanowitz J ( Department of Chemistry, University of Virginia, Charlottesville, Virginia 22901, USA); Hunt DF ( Department of Chemistry, University of Virginia, Charlottesville, Virginia 22901, USA); Olszewski NE ( Department of Plant Biology, University of Minnesota, Saint Paul, Minnesota 55108, USA); Sun TP ( Department of Biology, Duke University, Durham, North Carolina 27708, USA) |
| Abstract |
The DELLA family of transcription regulators functions as master growth repressors in plants by inhibiting phytohormone gibberellin (GA) signaling in response to developmental and environmental cues. DELLAs also play a central role in mediating cross-talk between GA and other signaling pathways via antagonistic direct interactions with key transcription factors. However, how these crucial protein-protein interactions can be dynamically regulated during plant development remains unclear. Here, we show that DELLAs are modified by the O-linked N-acetylglucosamine (O-GlcNAc) transferase (OGT) SECRET AGENT (SEC) in Arabidopsis. O-GlcNAcylation of the DELLA protein REPRESSOR OF ga1-3 (RGA) inhibits RGA binding to four of its interactors-PHYTOCHROME-INTERACTING FACTOR3 (PIF3), PIF4, JASMONATE-ZIM DOMAIN1, and BRASSINAZOLE-RESISTANT1 (BZR1)-that are key regulators in light, jasmonate, and brassinosteroid signaling pathways, respectively. Consistent with this, the sec-null mutant displayed reduced responses to GA and brassinosteroid and showed decreased expression of several common target genes of DELLAs, BZR1, and PIFs. Our results reveal a direct role of OGT in repressing DELLA activity and indicate that O-GlcNAcylation of DELLAs provides a fine-tuning mechanism in coordinating multiple signaling activities during plant development. |
