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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Grozdanovic, Milica Popovic, Milica Polovic, Natalija Burazer, Lidija Vuckovic, Olga Atanaskovic-Markovic, Marina Lindner, Buko Petersen, Arnd Gavrovic-Jankulovic, Marija |
| Description | Author Affiliation: Grozdanovic M ( Department of Biochemistry, Faculty of Chemistry, University of Belgrade, and Department of Allergology and Pulmonology, University Children's Hospital, Belgrade 11000, Serbia.) |
| Abstract | Actinidin, an abundant cysteine protease from kiwifruit, is a specific biomarker of isolated allergy to kiwifruit. This study evaluates the IgE-binding properties of biologically active and thermally inactivated actinidin. Employing two different activity assays (caseinolytic assay and zymogram with gelatin) we showed that actinidin obtained from kiwifruit extract under native conditions represents a mixture of inactive and active enzyme. The structural integrity of actinidin was confirmed by SDS-PAGE, Edman degradation, mass fingerprint and Western blot with polyclonal antibodies. Although it was capable of inducing positive skin prick test reactions, we failed to detect IgE reactivity of active actinidin in Western blot with patient sera. Thermally inactivated actinidin exhibited IgE reactivity both in vivo and in vitro, indicating that heat processed kiwifruit products may induce clinical reactivity. These findings imply that apart from the allergenic epitopes on its surface, actinidin also contains hidden epitopes inside the protein which become accessible to IgE upon thermal treatment. |
| File Format | HTM / HTML |
| ISSN | 02786915 |
| Issue Number | 3-4 |
| Volume Number | 50 |
| e-ISSN | 18736351 |
| Journal | Food and Chemical Toxicology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-03-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Toxicology__semicolon__nutritional Discipline Sciences Biological Markers Metabolism Cysteine Endopeptidases Food Hypersensitivity Fruit Immunology Immunoglobulin E Amino Acid Sequence Blotting, Western Drug Evaluation, Preclinical Electrophoresis, Gel, Two-dimensional Humans Molecular Sequence Data Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Toxicology Food Science |
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