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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Yang, Deng-Jye Chang, Yan-Zin Chen, Yi-Chen Liu, Shih-Chuan Hsu, Chuan-Hsiang Lin, Jau-Tien |
| Description | Country affiliation: Taiwan Author Affiliation: Yang DJ ( School of Health Diet and Industry Management and Department of Nutrition, Chung Shan Medical University and Chung Shan Medical University Hospital, 110, Section 1, Jianguo N. Road, Taichung 402, Taiwan.) |
| Abstract | The effects of scavenging 2, 2-diphenyl-2-picrylhydrazyl hydrate (DPPH) radicals and inhibiting low-density lipoprotein (LDL) oxidation, and phenolic quantities were used for the activity-guided separation to identify the effective components of litchi flower. The acetone extract of the flower with notable antioxidant capacities was suspended in water and sequentially partitioned with n-hexane, ethyl acetate (EA) and n-butanol. The EA partition with the highest phenolic levels and antioxidant capacities was subjected to silica gel column chromatography. Thirteen fractions (Fr. 1-13) were collected; Fr. 10-12 with higher phenolic levels and antioxidant effects were applied to Sephadex LH-20 column chromatography. Each fraction was further separated into three sub-fractions and the second ones (Fr. 10-II, 11-II, and 12-II) were the best, which two major compounds could be isolated by semi-preparative high performance liquid chromatography (HPLC). Through Mass (MS) and Nuclear Magnetic Resonance (NMR) measurements, they could be identified as (-)-epicatechin and proanthocyanidin A2. Their contents in the litchi flower were 5.52 and 11.12 mg/g of dry weight, respectively. The study was the first time to reveal the effective antioxidant components of litchi flower. |
| File Format | HTM / HTML |
| ISSN | 02786915 |
| Issue Number | 9 |
| Volume Number | 50 |
| e-ISSN | 18736351 |
| Journal | Food and Chemical Toxicology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-09-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Toxicology__semicolon__nutritional Discipline Sciences Antioxidants Pharmacology Flowers Chemistry Litchi Plant Extracts Chromatography, Gel Chromatography, Ion Exchange Magnetic Resonance Spectroscopy Mass Spectrometry Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Toxicology Food Science |
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