NDLI: Reducing blood glucose levels in TIDM mice with an orally administered extract of sericin from hIGF-I-transgenic silkworm cocoons.

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Reducing blood glucose levels in TIDM mice with an orally administered extract of sericin from hIGF-I-transgenic silkworm cocoons.

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Reducing blood glucose levels in TIDM mice with an orally administered extract of sericin from hIGF-I-transgenic silkworm cocoons.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Song, Zuowei Zhang, Mengyao Xue, Renyu Cao, Guangli Gong, Chengliang
Description	Author Affiliation: Song Z ( School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, PR China.); Zhang M ( School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, PR China.); Xue R ( School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, PR China); Cao G ( School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, PR China); Gong C ( School of Biology & Basic Medical Science, Soochow University, Suzhou 215123, PR China)
Abstract	In previous studies, we reported that the blood glucose levels of mice with type I diabetes mellitus (TIDM) was reduced with orally administered silk gland powder from silkworms transgenic for human insulin-like growth factor-I (hIGF-I). However, potential safety hazards could not be eliminated because the transgenic silk gland powder contained heterologous DNA, including the green fluorescent protein (gfp) and neomycin resistance (neo) genes. These shortcomings might be overcome if the recombinant hIGF-I were secreted into the sericin layer of the cocoon. In this study, silkworm eggs were transfected with a novel piggyBac transposon vector, pigA3GFP-serHS-hIGF-I-neo, containing the neo, gfp, and hIGF-I genes controlled by the sericin-1 (ser-1) promoter with the signal peptide DNA sequence of the fibrin heavy chain (Fib-H) and a helper plasmid containing the piggyBac transposase sequence under the control of the Bombyx mori actin 3 (A3) promoter, using sperm-mediated gene transfer to generate the transformed silkworms. The hIGF-I content estimated by enzyme-linked immunosorbent assay was approximately 162.7 ng/g. To estimate the biological activity of the expressed hIGF-I, streptozotocin-induced TIDM mice were orally administered sericin from the transgenic silkworm. The blood glucose levels of the mice were significantly reduced, suggesting that the extract from the transgenic hIGF-I silkworm cocoons can be used as an orally administered drug.
File Format	HTM / HTML
ISSN	02786915
Volume Number	67
e-ISSN	18736351
Journal	Food and Chemical Toxicology
Language	English
Publisher	Elsevier
Publisher Date	2014-05-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Toxicology__semicolon__nutritional Discipline Sciences Blood Glucose Analysis Bombyx Genetics Insulin-like Growth Factor I Sericins Pharmacology Administration, Oral Amino Acid Sequence Animals Animals, Genetically Modified Base Sequence Dna Primers Mice Molecular Sequence Data Polymerase Chain Reaction Administration & Dosage Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Toxicology Food Science

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Expression of the hIGF-I gene driven by the Fhx/P25 promoter in the silk glands of germline silkworm and transformed BmN cells

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The RGD sequence present in IGFBP-2 is required for reduced glucose clearance after oral glucose administration in female transgenic mice.

An active recombinant cocoonase from the silkworm Bombyx mori: bleaching, degumming and sericin degrading activities.

Analysis of transgenic silkworms producing insulin-like growth factor-I in Bombyx mori

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Proteomic analysis of sericin in Bombyx mori cocoons

Beneficial effects of Undaria pinnatifida ethanol extract on diet-induced-insulin resistance in C57BL/6J mice.

Reducing blood glucose levels in TIDM mice with an orally administered extract of sericin from hIGF-I-transgenic silkworm cocoons.

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