NDLI: Gene expression profiling identifies the zinc-finger protein Charlatan as a regulator of intestinal stem cells in Drosophila.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Amcheslavsky, Alla Nie, Yingchao Li, Qi He, Feng Tsuda, Leo Markstein, Michele Ip, Y. Tony
Description	Country affiliation: United States Author Affiliation: Amcheslavsky A ( Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.); Nie Y ( Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.); Li Q ( Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA.); He F ( Department of Microbiology and Physiological Systems, University of Massachusetts Medical School, Worcester, MA 01605, USA.); Tsuda L ( Animal Models of Aging, National Center for Geriatrics and Gerontology, Obu, Aichi 474-8511, Japan.); Markstein M ( Department of Biology, University of Massachusetts, Amherst, MA 01003, USA.); Ip YT ( Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA tony.ip@umassmed.edu.)
Abstract	Intestinal stem cells (ISCs) in the adult Drosophila midgut can respond to tissue damage and support repair. We used genetic manipulation to increase the number of ISC-like cells in the adult midgut and performed gene expression profiling to identify potential ISC regulators. A detailed analysis of one of these potential regulators, the zinc-finger protein Charlatan, was carried out. MARCM clonal analysis and RNAi in precursor cells showed that loss of Chn function caused severe ISC division defects, including loss of EdU incorporation, phosphorylated histone 3 staining and expression of the mitotic protein Cdc2. Loss of Charlatan also led to a much reduced histone acetylation staining in precursor cells. Both the histone acetylation and ISC division defects could be rescued by the simultaneous decrease of the Histone Deacetylase 2. The overexpression of Charlatan blocked differentiation reversibly, but loss of Charlatan did not lead to automatic differentiation. The results together suggest that Charlatan does not simply act as an anti-differentiation factor but instead functions to maintain a chromatin structure that is compatible with stem cell properties, including proliferation.
File Format	HTM / HTML
ISSN	09501991
e-ISSN	14779129
DOI	10.1242/dev.106237
Journal	Development
Issue Number	13
Volume Number	141
Language	English
Publisher	The Company of Biologists
Publisher Date	2014-07-01
Publisher Place	Great Britain (UK)
Access Restriction	Open
Subject Keyword	Discipline Developmental Discipline Biology Cell Differentiation Physiology Drosophila Proteins Drosophila Genetics Intestines Cytology Stem Cells Transcription Factors Animals Gene Expression Profiling Microarray Analysis Microscopy, Fluorescence Rna Interference Research Support, N.i.h., Extramural
Content Type	Text
Resource Type	Article
Subject	Developmental Biology Molecular Biology

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