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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Plummer, Nicholas W. Evsyukova, Irina Y. Robertson, Sabrina D. de Marchena, Jacqueline Tucker, Charles J. Jensen, Patricia |
| Description | Country affiliation: United States Author Affiliation: Plummer NW ( Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.); Evsyukova IY ( Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.); Robertson SD ( Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.); de Marchena J ( Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.); Tucker CJ ( Signal Transduction Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA.); Jensen P ( Neurobiology Laboratory, National Institute of Environmental Health Sciences, National Institutes of Health, Department of Health and Human Services, Research Triangle Park, NC 27709, USA patricia.jensen@nih.gov.) |
| Abstract | Investigating the developmental, structural and functional complexity of mammalian tissues and organs depends on identifying and gaining experimental access to diverse cell populations. Here, we describe a set of recombinase-responsive fluorescent indicator alleles in mice that significantly extends our ability to uncover cellular diversity by exploiting the intrinsic genetic signatures that uniquely define cell types. Using a recombinase-based intersectional strategy, these new alleles uniquely permit non-invasive labeling of cells defined by the overlap of up to three distinct gene expression domains. In response to different combinations of Cre, Flp and Dre recombinases, they express eGFP and/or tdTomato to allow the visualization of full cellular morphology. Here, we demonstrate the value of these features through a proof-of-principle analysis of the central noradrenergic system. We label previously inaccessible subpopulations of noradrenergic neurons to reveal details of their three-dimensional architecture and axon projection profiles. These new indicator alleles will provide experimental access to cell populations at unprecedented resolution, facilitating analysis of their developmental origin and anatomical, molecular and physiological properties. |
| File Format | HTM / HTML |
| ISSN | 09501991 |
| e-ISSN | 14779129 |
| Journal | Development |
| Issue Number | 24 |
| Volume Number | 142 |
| Language | English |
| Publisher | The Company of Biologists |
| Publisher Date | 2015-12-15 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | Open |
| Subject Keyword | Discipline Developmental Discipline Biology Neurons Cytology Recombinases Metabolism Staining And Labeling Alleles Animals Axons Fluorescent Dyes Gene Expression Green Fluorescent Proteins Mice Research Support, N.i.h., Intramural |
| Content Type | Text |
| Resource Type | Article |
| Subject | Developmental Biology Molecular Biology |
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