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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Malet, Arnaud Faure, Marie-Odile Deletage, Nathalie Pereira, Bruno Haas, Jerome Lambert, Gregory |
| Description | Country affiliation: France Author Affiliation: Malet A ( From the *Scientific Affairs, Septodont, Saint Maur des Fosses, France) |
| Abstract | BACKGROUND: Although local anesthetics (LAs) are generally accepted as being safe, incidental neuronal damage has been reported for all LAs in humans. Therefore, in this study, we compared the dose corresponding to 50% cell lethality (LD50) of articaine, lidocaine, mepivacaine, bupivacaine, prilocaine, and ropivacaine in human neuroblastoma cells. METHODS: Undifferentiated SH-SY5Y cells were exposed for 20 minutes to different concentrations of each LA. Metabolic activity of viable cells was assessed by a cell viability test with a tetrazolium dye (WST-1) followed by optical density quantification. LD50 was determined by extrapolation of curve response. RESULTS: As expected, all LAs induced cell death in a concentration-dependent manner. The bupivacaine, lidocaine, prilocaine, mepivacaine, articaine, and ropivacaine LD50 were 0.95 ± 0.08, 3.35 ± 0.33, 4.32 ± 0.39, 4.84 ± 1.28, 8.98 ± 2.07, and 13.43 ± 0.61 mM, respectively, after 20 minutes of incubation on SH-SY5Y cells. Ropivacaine LD50 was significantly different from the bupivacaine, lidocaine, mepivacaine, and prilocaine LD50 (all P ≤ 0.009). No significant difference was obtained between ropivacaine and articaine LD50 and between prilocaine, lidocaine, and mepivacaine LD50. Articaine LD50 was significantly different from lidocaine LD50 (P = 0.03). Bupivacaine LD50 was significantly lower compared with all LAs (all P ≤ 0.003). CONCLUSIONS: LA neurotoxicity was tested in a validated in vitro model SH-SY5Y, a human neuroblastoma cell line. Three groups of LAs were identified in terms of toxicity: (1) the less (ropivacaine, articaine); (2) medium (mepivacaine, prilocaine, lidocaine); and (3) the high (bupivacaine). Among dental anesthetics, articaine is the least neurotoxic in SH-SY5Y cells. |
| File Format | HTM / HTML |
| ISSN | 00032999 |
| Issue Number | 3 |
| Volume Number | 120 |
| e-ISSN | 15267598 |
| Journal | Anesthesia & Analgesia |
| Language | English |
| Publisher | Lippincott Williams & Wilkins |
| Publisher Date | 2015-03-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Anesthesiology Anesthetics, Local Toxicity Neuroblastoma Pathology Neurotoxicity Syndromes Etiology Cell Line, Tumor Cell Shape Drug Effects Cell Survival Dose-response Relationship, Drug Humans Inhibitory Concentration 50 Risk Assessment Time Factors Comparative Study Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Anesthesiology and Pain Medicine |
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