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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lê, H. Q. A. Sauriat-Dorizon, H. Korri-Youssoufi, H. |
| Description | Country affiliation: France Author Affiliation: Lê HQ ( Equipe de Chimie Bioorganique et Bioinorganique, CNRS UMR 8182, Institut de Chimie Moléculaire et de Matériaux d'Orsay, Université Paris-Sud, Bâtiment 420, 91405 Orsay, France.) |
| Abstract | An electrochemical label-free immunosensor based on a biotinylated single-chain variable fragment (Sc-Fv) antibody immobilized on copolypyrrole film is described. An efficient immunosensor device formed by immobilization of a biotinylated single-chain antibody on an electropolymerized copolymer film of polypyrrole using biotin/streptavidin system has been demonstrated for the first time. The response of the biosensor toward antigen detection was monitored by surface plasmon resonance (SPR) and electrochemical analysis of the polypyrrole response by differential pulse voltammetry (DPV). The composition of the copolymer formed from a mixture of pyrrole (py) as spacer and a pyrrole bearing a N-hydroxyphthalimidyl ester group on its 3-position (pyNHP), acting as agent linker for biomolecule immobilization, was optimized for an efficient immunosensor device. The ratio of py:pyNHP for copolymer formation was studied with respect to the antibody immobilization and antigen detection. SPR was employed to monitor in real time the electropolymerization process as well as the step-by-step construction of the biosensor. FT-IR demonstrates the chemical copolymer composition and the efficiency of the covalent attachment of biomolecules. The film morphology was analyzed by electron scanning microscopy (SEM). Results show that a well organized layer is obtained after Sc-Fv antibody immobilization thanks to the copolymer composition defined with optimized pyrrole and functionalized pyrrole leading to high and intense redox signal of the polypyrrole layer obtained by the DPV method. Detection of specific antigen was demonstrated by both SPR and DPV, and a low concentration of 1 pg mL(-1) was detected by measuring the variation of the redox signal of polypyrrole. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Issue Number | 1 |
| Volume Number | 674 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-07-26 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Antigens Analysis Chemistry Polymers Pyrroles Single-chain Antibodies Immunology Surface Plasmon Resonance Methods Antigen-antibody Reactions Biotinylation Electrochemistry Immunoassay Journal Article Research Support, Non-u.s. Gov't Review |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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