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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Selvakumar, L. S. Thakur, M. S. |
| Description | Country affiliation: India Author Affiliation: Selvakumar LS ( Fermentation Technology and Bioengineering Department, Central Food Technological Research Institute, Mysore 570020, Karnataka, India.) |
| Abstract | In this article, we describe a dipstick based immunochemiluminescence (immuno-CL) biosensor for the detection of vitamin B(12) in energy drinks. The method is a direct competitive type format involving the immobilization of vitamin B(12) antibody on nitrocellulose membrane (NC) followed by treatment with vitamin B(12) and vitamin B(12)-alkaline phosphatase conjugate to facilitate the competitive binding. The dipstick was further treated with substrate disodium 2-chloro-5-(4-methoxyspiro {1,2-dioxetane-3,2¢-(5¢-chloro)tricyclo[3.3.1.13,7]decan}-4-yl)-1-phenyl phosphate (CDP-Star) to generate chemiluminescence (CL). The number of photons generated was inversely proportional to the vitamin B(12) concentration. After systematic optimization, the limit of detection was 1 ng mL(-1). The coefficient of variation was below 0.2% for both intra- and inter-assay precision. Vitamin B(12) was extracted from energy drinks with recovery ranged from 90 to 99.4%. Two different energy drinks samples were analyzed, and a good correlation was observed when the data were compared with a reference enzyme linked immuno sorbent assay (ELISA) method. The developed method is suitable for an accurate, sensitive, and high-throughput screening of vitamin B(12) in energy drinks samples. The dipstick technique based on immuno-CL is suitable for the detection of several analyte in food and environmental samples. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 722 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-04-13 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Biosensing Techniques Energy Drinks Analysis Vitamin B 12 Alkaline Phosphatase Chemistry Metabolism Antibodies, Immobilized Immunology Collodion Haptens Immunoassay Luminescent Measurements Isolation & Purification Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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