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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Choi, Hyung Woo Sakata, Yasuhiko Kurihara, Yoshikazu Ooya, Tooru Takeuchi, Toshifumi |
| Description | Country affiliation: Japan Author Affiliation: Choi HW ( Graduate School of Engineering, Kobe University, Nada-ku, Kobe, Japan.) |
| Abstract | Reflectometric interference spectroscopy (RIfS) is a label-free, time-resolved technique, and suitable for detecting antibody-antigen interaction. This work describes a continuous flow biosensor for C-reactive protein (CRP), involving an effective immobilization method of a monoclonal antibody against CRP (anti-CRP) to achieve highly sensitive RIfS-based detection of CRP. The silicon nitride-coated silicon chip (SiN chip) for the RIfS sensing was first treated with trimethylsilylchloride (TMS), followed by UV-light irradiation to in situ generation of homogeneous silanols on the surface. Following amination by 3-aminopropyltriethoxysilane, carboxymethyldextran (CMD) was grafted, and subsequently, protein A was immobilized to create the oriented anti-CRP surface. The immobilization process of protein A and anti-CRP was monitored with the RIfS system by consecutive injections of an amine coupling reagent, protein A and anti-CRP, respectively, to confirm the progress of each step in real time. The sensitivity was enhanced when all of the processes were adopted, suggesting that the oriented immobilization of anti-CRP via protein A that was coupled with the grafted CMD on the aminated surface of TMS-treated SiN chip. The feasibility of the present sensing system was demonstrated on the detection of CRP, where the silicon-based inexpensive chips and the simple optical setup were employed. It can be applied to other target molecules in various fields of life science as a substitute of surface plasmon resonance-based expensive sensors. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 728 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-05-30 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Antibodies, Immobilized Chemistry Biosensing Techniques Instrumentation C-reactive Protein Analysis Protein Array Analysis Silanes Amination Animals Immunology Equipment Design Humans Immobilized Proteins Sensitivity And Specificity Spectrum Analysis Staphylococcal Protein A Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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