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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Fan, Jiangli Liu, Xiaojian Hu, Mingming Zhu, Hao Song, Fengling Peng, Xiaojun |
| Description | Country affiliation: China Author Affiliation: Fan J ( State Key Laboratory of Fine Chemicals, Dalian University of Technology, No. 2 Linggong Road, High-tech District, Dalian 116024, China. fanjl@dlut.edu.cn) |
| Abstract | Based on a boron dipyrromethene (BODIPY) derivative containing an N, O and S tridentate ligand, a Cu(2+) fluorescent probe BTCu was developed. The detection mechanism was verified as Cu(2+)-promoted oxidative dehydrogenation of an amine moiety, leading to a formation of a fluorescent Cu(+)-Schiff base complex. Free BTCu exhibited a maximum absorption wavelength at 496 nm, and a very weak maximum emission at 511 nm. Upon addition of various metals ions, it showed large fluorescence enhancement toward Cu(2+) (417-fold in MeCN and 103-fold in MeCN/HEPES solution, respectively) with high selectivity. The detection limits are as low as 1.74×10(-8) M and 4.96×10(-8) M in the two different solutions, respectively. And BTCu could work in a wide pH range with an extraordinary low pK(a) of 1.21±0.06. Using fluorescence microscopy, the probe was shown to be capable of penetrating into living cells and imaging intracellular Cu(2+) changes. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 735 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-07-20 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Boron Compounds Chemistry Copper Analysis Fluorescent Dyes Intracellular Space Microscopy, Fluorescence Methods Cations, Divalent Hela Cells Humans Limit Of Detection Oxidation-reduction Schiff Bases Spectrometry, Fluorescence Evaluation Studies Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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