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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Özyürek, Mustafa Baki, Sefa Güngör, Nilay Çelik, S. Esin Güçlü, Kubilay Apak, Resat |
| Description | Country affiliation: Turkey Author Affiliation: Özyürek M ( Department of Chemistry, Faculty of Engineering, Istanbul University, Avcilar 34320, Istanbul, Turkey.) |
| Abstract | A novel on-line HPLC-DTNB method was developed for the selective determination of biologically important thiols (biothiols) such as L-cysteine (Cys), glutathione (GSH), homocysteine (HCys), N-acetylcysteine (NAC), and 1,4-dithioerythritol (DTE) in pharmaceuticals and tissue homogenates. The biothiols were separated on C18 column using gradient elution, reacted with the postcolumn reagent, DTNB in 0.5% M-ß-CD (w/v) solution at pH 8, to form yellow-colored 5-thio-2-nitrobenzoic acid (TNB), and monitored with a PDA detector (λ=410 nm). With the optimized conditions for chromatography and the post-column derivatization, 40 nM of NAC, 40 nM of Cys, and 50 nM of GSH can be determined. The relative standard deviations of the recommended method were in the range of 3.2-5.4% for 50 µM biothiols. The negative peaks of biothiol constituents were monitored by measuring the increase in absorbance due to TNB chromophore. The detection limits of biothiols at 410 nm (in the range of 0.04-0.58 µM) after post-column derivatization with DTNB+M-ß-CD were much lower than those at 205 nm UV-detection without derivatization, and were distinctly lower than those with post-column DTNB alone. The method is rapid, inexpensive, versatile, nonlaborious, uses stable reagents, and enables the on-line qualitative and quantitative estimation of biothiol constituents of biological fluids and pharmaceuticals. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 750 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-10-31 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Chromatography, High Pressure Liquid Dithionitrobenzoic Acid Chemistry Sulfhydryl Compounds Analysis Cysteine Analogs & Derivatives Dithioerythritol Glutathione Oligosaccharides Pharmaceutical Preparations Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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