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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Beleites, Claudia Neugebauer, Ute Bocklitz, Thomas Krafft, Christoph Popp, Jürgen |
| Description | Country affiliation: Germany Author Affiliation: Beleites C ( Department of Spectroscopy and Imaging, Institute of Photonic Technology, Jena, Germany. Claudia.Beleites@ipht-jena.de) |
| Abstract | In biospectroscopy, suitably annotated and statistically independent samples (e.g. patients, batches, etc.) for classifier training and testing are scarce and costly. Learning curves show the model performance as function of the training sample size and can help to determine the sample size needed to train good classifiers. However, building a good model is actually not enough: the performance must also be proven. We discuss learning curves for typical small sample size situations with 5-25 independent samples per class. Although the classification models achieve acceptable performance, the learning curve can be completely masked by the random testing uncertainty due to the equally limited test sample size. In consequence, we determine test sample sizes necessary to achieve reasonable precision in the validation and find that 75-100 samples will usually be needed to test a good but not perfect classifier. Such a data set will then allow refined sample size planning on the basis of the achieved performance. We also demonstrate how to calculate necessary sample sizes in order to show the superiority of one classifier over another: this often requires hundreds of statistically independent test samples or is even theoretically impossible. We demonstrate our findings with a data set of ca. 2550 Raman spectra of single cells (five classes: erythrocytes, leukocytes and three tumour cell lines BT-20, MCF-7 and OCI-AML3) as well as by an extensive simulation that allows precise determination of the actual performance of the models in question. |
| File Format | HTM / HTML |
| ISSN | 00032670 |
| Volume Number | 760 |
| e-ISSN | 18734324 |
| Journal | Analytica Chimica Acta |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-01-14 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Discipline Chemistry Models, Theoretical Cells, Cultured Erythrocytes Chemistry Classification Cytology Humans Leukocytes Mcf-7 Cells Sample Size Spectrum Analysis, Raman Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Spectroscopy Environmental Chemistry Analytical Chemistry Biochemistry |
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